Mouse Platelet Derived Growth Factor C ELISA Kit | PDGFC elisa kit

Mouse Platelet Derived Growth Factor C ELISA Kit

Cat. Num. AAA763452

• Gene Names: PDGFC; SCDGF; FALLOTEIN
• Reactivity: Mouse
• Synonyms: Platelet Derived Growth Factor C; Mouse Platelet Derived Growth Factor C ELISA Kit; PDGFC/FALLOTEIN/Fallotein/fallotein/PDGF-C/platelet derived growth factor C/platelet-derived growth factor C/SCDGFSpinal cord-derived growth factor/secretory growth factor-like protein; PDGFC elisa kit

• Reactivity: Mouse
• Sequence Length: 345

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PDGFC elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse PDGFC antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse PDGFC antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PDGFC amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse PDGFC can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 9994187
• NCBI GeneID: 56034
• NCBI Accession #: NP_057289.1
• NCBI GenBank Nucleotide #: NM_016205.2
• Molecular Weight: 20,609 Da
• NCBI Official Full Name: platelet-derived growth factor C
• NCBI Official Synonym Full Names: platelet derived growth factor C
• NCBI Official Symbol: PDGFC
• NCBI Official Synonym Symbols: SCDGF; FALLOTEIN
• NCBI Protein Information: platelet-derived growth factor C
• UniProt Protein Name: Platelet-derived growth factor C
• Protein Family: Platelet-derived growth factor
• UniProt Gene Name: PDGFC
• UniProt Synonym Gene Names: SCDGF; PDGF-C; SCDGF; PDGFC latent form; PDGFC receptor-binding form
• UniProt Entry Name: PDGFC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines. This gene product appears to form only homodimers. It differs from the platelet-derived growth factor alpha and beta polypeptides in having an unusual N-terminal domain, the CUB domain. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2010]

Uniprot Description

PDGFC: Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen and chemoattractant for cells of mesenchymal origin. Required for normal skeleton formation during embryonic development, especially for normal development of the craniofacial skeleton and for normal development of the palate. Required for normal skin morphogenesis during embryonic development. Plays an important role in wound healing, where it appears to be involved in three stages: inflammation, proliferation and remodeling. Plays an important role in angiogenesis and blood vessel development. Involved in fibrotic processes, in which transformation of interstitial fibroblasts into myofibroblasts plus collagen deposition occurs. The CUB domain has mitogenic activity in coronary artery smooth muscle cells, suggesting a role beyond the maintenance of the latency of the PDGF domain. In the nucleus, PDGFC seems to have additional function. Belongs to the PDGF/VEGF growth factor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q32

Cellular Component: cell surface; cytoplasm; cytosol; endoplasmic reticulum lumen; extracellular region; extracellular space; Golgi membrane; nucleus; plasma membrane

Molecular Function: growth factor activity; platelet-derived growth factor receptor binding; protein binding; protein homodimerization activity

Biological Process: central nervous system development; embryonic development; gut development; organ morphogenesis; platelet-derived growth factor receptor signaling pathway; positive regulation of cell division; positive regulation of cell migration; positive regulation of DNA replication; positive regulation of fibroblast proliferation; positive regulation of MAP kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid autophosphorylation; regulation of peptidyl-tyrosine phosphorylation; transmembrane receptor protein tyrosine kinase activation (dimerization)