Phosphodiesterase 6D cGMP-Specific Rod Delta Recombinant Protein | PDE6D recombinant protein

Recombinant Human Phosphodiesterase 6D cGMP-Specific Rod Delta

Cat. Num. AAA143850

• Gene Names: PDE6D; PDED; JBTS22
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Phosphodiesterase 6D cGMP-Specific Rod Delta; Recombinant Human Phosphodiesterase 6D cGMP-Specific Rod Delta; PDE6D Human; Phosphodiesterase 6D cGMP-Specific Rod Delta Human Recombinant; Retinal rod rhodopsin-sensitive cGMP 3''; 5''-cyclic phosphodiesterase subunit delta; GMP-PDE delta; Protein p17; PDE6D; PDED; PDE6D recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The PDE6D protein solution contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 1mM DTT and 100mM NaCl.; Sterile filtered colorless solution.
• Sequence: MSAKDERARE ILRGFKLNWM NLRDAETGKI LWQGTEDLSV PGVEHEARVP KKILKCKAVS RELNFSSTEQ MEKFRLEQKV YFKGQCLEEW FFEFGFVIPN STNTWQSLIE AAPESQMMPA SVLTGNVIIE TKFFDDDLLV STSRVRLFYV GSHHHHHH
• Sequence Length: 93

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

Related Product Information for PDE6D recombinant protein

Description: PDE6D produced in E Coli is a single, non-glycosylated polypeptide chain containing 158 amino acids (1-150 a.a.) and having a molecular mass of 18.4kDa. PDE6D is fused to an 8 amino acids His Tag at C-terminus and purified by proprietary chromatographic techniques.

Introduction: Human PDE6D was initially identified as a fourth subunit of rod-specific cGMP phosphodiesterase, PDE6. PDE6D is an Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain. PDE6D interacts with RPGR, ARL2 and ARL3. The catalytically active PDE6 is a heterodimer (alphabeta) that is controlled by two inhibitory gamma subunits. Since PDE6D does not change the catalytic properties of PDEalphabeta, its function is still unanswered. PDE6D is expressed specifically in the retina.

Product Categories/Family for PDE6D recombinant protein

Enzymes; Esterase

NCBI and Uniprot Product Information

• NCBI GI #: 597518021
• NCBI GeneID: 5147
• NCBI Accession #: NP_001277947.1
• NCBI GenBank Nucleotide #: NM_001291018.1
• UniProt Accession #: O43924; O43250
• Molecular Weight: 17,420 Da
• NCBI Official Full Name: retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta isoform 2
• NCBI Official Synonym Full Names: phosphodiesterase 6D, cGMP-specific, rod, delta
• NCBI Official Symbol: PDE6D
• NCBI Official Synonym Symbols: PDED; JBTS22
• NCBI Protein Information: retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta; GMP-PDE delta; protein p17
• UniProt Protein Name: Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase subunit delta
• Protein Family: Retinal rod rhodopsin-sensitive cGMP 3',5'-cyclic phosphodiesterase
• UniProt Gene Name: PDE6D
• UniProt Synonym Gene Names: PDED; GMP-PDE delta
• UniProt Entry Name: PDE6D_HUMAN

NCBI Description

This gene encodes the delta subunit of rod-specific photoreceptor phosphodiesterase (PDE), a key enzyme in the phototransduction cascade. A similar protein in cow functions in solubilizing membrane-bound PDE. In addition to its role in the PDE complex, the encoded protein is thought to bind to prenyl groups of proteins to target them to subcellular organelles called cilia. Mutations in this gene are associated with Joubert syndrome-22. Alternative splicing results in multiple splice variants. [provided by RefSeq, Mar 2014]

Uniprot Description

PDE6D: Acts as a GTP specific dissociation inhibitor (GDI). Increases the affinity of ARL3 for GTP by several orders of magnitude and does so by decreasing the nucleotide dissociation rate. Stabilizes Arl3-GTP by decreasing the nucleotide dissociation. Belongs to the PDE6D/unc-119 family.

Chromosomal Location of Human Ortholog: 2q35-q36

Cellular Component: cytoskeleton; cytoplasmic vesicle membrane; cytoplasmic vesicle; cytosol

Molecular Function: protein binding; 3',5'-cyclic-nucleotide phosphodiesterase activity; Rab GTPase binding; GTPase inhibitor activity

Biological Process: visual perception; metabolic process; organelle organization and biogenesis; negative regulation of catalytic activity; response to stimulus

Disease: Joubert Syndrome 22