Human programmed cell death 1 ELISA Kit | PDCD1 elisa kit

Human Programmed Death 1, PD-1 ELISA Kit

Cat. Num. AAA700258

• Gene Names: PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• Reactivity: Human
• Synonyms: programmed cell death 1; Human Programmed Death 1; PD-1 ELISA Kit; Human Programmed Death 1 (PD-1) ELISA KIT; CD279; PD1; SLEB2; hPD-1; hPD-l; ; PDCD1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat PCT. No significant cross-reactivity or interference between rat PCT and analogues was observed.
• Sequence Length: 288

• Samples: Serum, Plasma, Tissue Homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25 pg/ml-2000 pg/ml
• Sensitivity: 7.8 pg/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDCD1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PCT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PCT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCT bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 167857792
• NCBI GeneID: 5133
• NCBI Accession #: NP_005009.2
• NCBI GenBank Nucleotide #: NM_005018.2
• UniProt Accession #: Q15116; O00517; Q8IX89
• Molecular Weight: 31,647 Da
• NCBI Official Full Name: programmed cell death protein 1
• NCBI Official Synonym Full Names: programmed cell death 1
• NCBI Official Symbol: PDCD1
• NCBI Official Synonym Symbols: PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1
• NCBI Protein Information: programmed cell death protein 1; protein PD-1; systemic lupus erythematosus susceptibility 2
• UniProt Protein Name: Programmed cell death protein 1
• Protein Family: Programmed cell death
• UniProt Gene Name: PDCD1
• UniProt Synonym Gene Names: PD1; Protein PD-1; hPD-1
• UniProt Entry Name: PDCD1_HUMAN

NCBI Description

This gene encodes a cell surface membrane protein of the immunoglobulin superfamily. This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Inhibitory cell surface receptor involved in the regulation of T-cell function during immunity and tolerance. Upon ligand binding, inhibits T-cell effector functions in an antigen-specific manner. Possible cell death inducer, in association with other factors.1 PublicationManual assertion based on experiment in:Ref.9