PD-1 ELISA Pair Set | PDCD1 elisa pair set

PD-1 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118576

• Synonyms: PD-1; PD-1 Matched ELISA Antibody Pair Set; Human; Human PD-1 ELISA Pair Set; programmed cell death 1; CD279 Matched ELISA Antibody Pair Set; hPD-1 Matched ELISA Antibody Pair Set; hPD-l Matched ELISA Antibody Pair Set; hSLE1 Matched ELISA Antibody Pair Set; PD1 Matched ELISA Antibody Pair Set; SLEB2 Matched ELISA Antibody Pair Set; PDCD1 elisa pair set

• Specificity: Quantitative determination of Human PD-1

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 3.91-250pg/mL
• Sensitivity: 3.91pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of mouse anti-Human PD-1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of mouse anti-Human PD-1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.3ug/mL in Dilution Buffer before use. ; 3. Standard Each vial contains 57 ng of recombinant Human PD-1. Reconstitute with 1 mL Dilution Buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in Dilution Buffer, and a high standard of 250 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for PDCD1 elisa pair set

Programmed cell death 1, also known as PDCD1, is a type I transmembrane glycoprotein, and is an immunoreceptor belonging to the CD28/CTLA-4 family negatively regulates antigen receptor signaling by recruiting protein tyrosine phosphatase, SHP-2 upon interacting with either of two ligands, PD-L1 or PD-L2. PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-gamma by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. Furthermore, PD1 is shown to be a regulator of virus-specific CD8+ T cell survival in HIV infection. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.

This PD-1 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human PD-1. It contains Human PD-1 capture antibody, Human PD-1 detector antibody (HRP) and a highly purified recombinant Human PD-1 protein.

Product Categories/Family for PDCD1 elisa pair set

ITIM/ITAM Immunoreceptors and Related Molecules Cancer Drug Targets

References

James ES, et al. (2005) PDCD1: a tissue-specific susceptibility locus for inherited inflammatory disorders. Genes Immun. 6(5): 430-7.Okazaki T, et al. (2007) PD-1 and PD-1 ligands: from discovery to clinical application. Int Immunol. 19(7): 813-24.del Rio ML, et al. (2008) PD-1/PD-L1, PD-1/PD-L2, and other co-inhibitory signaling pathways in transplantation. Transpl Int. 21(11): 1015-28.Riley JL.(2009) PD-1 signaling in primary T cells. Immunol Rev. 229(1): 114-25.