Typical Testing Data/Standard Curve (for reference only)
Porcine PDGFA Associated Protein 1 ELISA Kit | PDAP1 elisa kit
Porcine PDGFA Associated Protein 1 ELISA Kit
Gene Names
PDAP1; PAP; PAP1; HASPP28
Reactivity
Porcine
Synonyms
PDGFA Associated Protein 1; Porcine PDGFA Associated Protein 1 ELISA Kit; PDAP1 elisa kit
Reactivity
Porcine
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PDAP1 elisa kit
Intended Uses: This PDAP1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine PDAP1. This ELISA kit for research use only!
Principle of the Assay: PDAP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PDAP1 antibody and an PDAP1-HRP conjugate. The assay sample and buffer are incubated together with PDAP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDAP1 concentration since PDAP1 from samples and PDAP1-HRP conjugate compete for the anti-PDAP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PDAP1 from the sample, fewer sites are left to bind PDAP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDAP1 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: PDAP1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PDAP1 antibody and an PDAP1-HRP conjugate. The assay sample and buffer are incubated together with PDAP1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PDAP1 concentration since PDAP1 from samples and PDAP1-HRP conjugate compete for the anti-PDAP1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PDAP1 from the sample, fewer sites are left to bind PDAP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PDAP1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
20,630 Da
NCBI Official Full Name
PDGFA associated protein 1, isoform CRA_b
NCBI Official Synonym Full Names
PDGFA associated protein 1
NCBI Official Symbol
PDAP1
NCBI Official Synonym Symbols
PAP; PAP1; HASPP28
NCBI Protein Information
28 kDa heat- and acid-stable phosphoprotein; PDGF-associated protein
UniProt Protein Name
28 kDa heat- and acid-stable phosphoprotein
Protein Family
UniProt Gene Name
PDAP1
UniProt Synonym Gene Names
HASPP28; PAP; PAP1
UniProt Entry Name
HAP28_HUMAN
NCBI Description
The protein encoded by this gene is a phosphoprotein that may upregulate the PDGFA-stimulated growth of fibroblasts and also downregulate the mitogenicity of PDGFB. The encoded protein in rodents has been shown to bind PDGFA with a low affinity. [provided by RefSeq, Dec 2010]
Uniprot Description
PDAP1: Enhances PDGFA-stimulated cell growth in fibroblasts, but inhibits the mitogenic effect of PDGFB. Belongs to the PDAP1 family.
Chromosomal Location of Human Ortholog: 7q22.1
Biological Process: cell proliferation; signal transduction
Similar Products
Product Notes
The Porcine PDAP1 pdap1 (Catalog #AAA755233) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA755233 ELISA Kit recognizes Porcine PDAP1. It is sometimes possible for the material contained within the vial of "PDGFA Associated Protein 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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