PCNA recombinant protein

PCNA, Recombinant, Human (Proliferating Cell Nuclear Antigen)

Cat. Num. AAA638232

• Purity: Highly Purified; 95% by SDS-PAGE.
• Synonyms: PCNA; Recombinant; Human (Proliferating Cell Nuclear Antigen); PCNA recombinant protein

• Host: Sf9 insect cells
• Purity/Purification: Highly Purified; 95% by SDS-PAGE.
• Form/Format: Supplied as a liquid in 16mM HEPES buffer, pH 8, 400mM sodium chloride, 20% glycerol.

• Application Notes: Suitable for use in ELISA and Western Blot with monoclonal anti-hexa-His-tag antibody and Scleroderma patient sera. Suitaable for biotinylation and iodination.; Dilution: ELISA: 0.5-1.1ug/ml; Optimal dilution determined by the researcher.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for PCNA recombinant protein

Proliferating cell nuclear antigen (PCNA), a protein of 30kD molecular weight also known as cyclin, forms a trimer ring around a DNA double-helix. It binds to a variety of other nuclear proteins and thereby organises biochemical processes at the DNA replication fork. PCNA thereby assumes important functions in DNA synthesis, repair and processing, the regulation of cell cycle and even apoptosis. Anti-PCNA antibodies occur in systemic lupus, where they represent a rare specificity (< 10 % of SLE patients). However, their presence alone or in combination with other autoantibodies should alert the clinician to the presence of defined or evolving SLE.

PCNA Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 29.7kD. PCNA is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Product Categories/Family for PCNA recombinant protein

Molecular Biology; MB-Nuclear Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 49456555
• NCBI GeneID: 5111
• UniProt Accession #: Q6FHF5; P12004
• Molecular Weight: 28,706 Da
• NCBI Official Full Name: PCNA
• NCBI Official Synonym Full Names: proliferating cell nuclear antigen
• NCBI Official Symbol: PCNA
• NCBI Protein Information: proliferating cell nuclear antigen; cyclin; DNA polymerase delta auxiliary protein
• UniProt Protein Name: Proliferating cell nuclear antigen
• Protein Family: PCNA-associated factor
• UniProt Gene Name: PCNA
• UniProt Entry Name: Q6FHF5_HUMAN

NCBI Description

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

Uniprot Description

PCNA: This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Homotrimer. Forms a complex with activator 1 heteropentamer in the presence of ATP. Interacts with EXO1, POLH, POLK, DNMT1, ERCC5, FEN1, CDC6 and POLDIP2. Interacts with APEX2; this interaction is triggered by reactive oxygen species and increased by misincorporation of uracil in nuclear DNA. Forms a ternary complex with DNTTIP2 and core histone. Interacts with KCTD10 and PPP1R15A. Interacts with POLD1, POLD3 and POLD4. Interacts with BAZ1B; the interaction is direct. Interacts with HLTF and SHPRH. Interacts with NUDT15. Interaction is disrupted in response to UV irradiation and acetylation. Interacts with p21Cip1/p21(CIP1) and CDT1; interacts via their PIP-box which also recruits the DCX(DTL) complex. Interacts with DDX11. Interacts with EGFR; positively regulates PCNA. Interacts with C12orf48/PARI. Interacts with SMARCAD1. Belongs to the PCNA family.

Protein type: DNA replication; Cell cycle regulation

Chromosomal Location of Human Ortholog: 20pter-p12

Cellular Component: nucleoplasm; centrosome; PCNA complex; cytoplasm; nuclear replication fork; DNA replication factor C complex; nucleus

Molecular Function: identical protein binding; protein binding; DNA polymerase processivity factor activity; purine-specific mismatch base pair DNA N-glycosylase activity; MutLalpha complex binding; dinucleotide insertion or deletion binding; receptor tyrosine kinase binding

Biological Process: mismatch repair; heart development; positive regulation of deoxyribonuclease activity; DNA strand elongation during DNA replication; DNA repair; response to lipid; leading strand elongation; G1/S-specific transcription in mitotic cell cycle; telomere maintenance via semi-conservative replication; cell proliferation; bypass DNA synthesis; response to cadmium ion; epithelial cell differentiation; base-excision repair; nucleotide-excision repair; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; telomere maintenance; G1/S transition of mitotic cell cycle; regulation of DNA replication

Disease: Ataxia-telangiectasia-like Disorder 2