Protocadherin 15 Recombinant Protein | PCDH15 recombinant protein

Recombinant Protocadherin 15 (PCDH15)

Cat. Num. AAA2033494

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Protocadherin 15; Recombinant Protocadherin 15 (PCDH15); USH1F; DFNB23; CDHR15; Deafness; Autosomal Recessive 23; Cadherin-Related Family Member 15; PCDH15 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: PATIVAIDEESRNGTI LVDNMLIKGT AGGPDPTIEL SLKDNVDYWV LLDPVKQMLFLNSTGRVLDR DPPMNIHSIV VQVQCVNKKV GTVIYHEVRI VVRDRNDNSPTFKHESYYAT VNELTPVGTT IFTGFSGDNG ATDIDDGPNG QIEYVIQYNPEDPTSNDTFE IPLMLTGNVV LRKRLNYEDK TRYYVIIQAN DRAQNLNERRTTTTTLTVDV LDGDDLGPMF
• Sequence Length: 1955

• Applicable Applications for PCDH15 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 29.0kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Pro45~Phe270
• Organism Species: Mouse
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 14581464
• Molecular Weight: 185,964 Da
• NCBI Official Full Name: protocadherin 15
• UniProt Protein Name: Protocadherin-15
• Protein Family: Protocadherin
• UniProt Gene Name: PCDH15
• UniProt Synonym Gene Names: USH1F
• UniProt Entry Name: PCD15_HUMAN

Uniprot Description

PCDH15: Calcium-dependent cell-adhesion protein. Essential for maintenance of normal retinal and cochlear function. Defects in PCDH15 are the cause of Usher syndrome type 1F (USH1F). USH is a genetically heterogeneous condition characterized by the association of retinitis pigmentosa and sensorineural deafness. Age at onset and differences in auditory and vestibular function distinguish Usher syndrome type 1 (USH1), Usher syndrome type 2 (USH2) and Usher syndrome type 3 (USH3). USH1 is characterized by profound congenital sensorineural deafness, absent vestibular function and prepubertal onset of progressive retinitis pigmentosa leading to blindness. Defects in PCDH15 are a cause of Usher syndrome type 1D/F (USH1DF). USH1DF patients are heterozygous for mutations in CDH23 and PCDH15, indicating a digenic inheritance pattern. Defects in PCDH15 are the cause of deafness autosomal recessive type 23 (DFNB23). DFNB23 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Cell development/differentiation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q21.1

Cellular Component: integral to plasma membrane; photoreceptor outer segment; stereocilium; synapse

Biological Process: cell adhesion; cell-cell signaling; equilibrioception; nervous system development; photoreceptor cell maintenance; sensory perception of light stimulus; sensory perception of sound

Disease: Deafness, Autosomal Recessive 23; Usher Syndrome, Type Id; Usher Syndrome, Type If