PBK recombinant protein

PBK, 1-322aa, Human, His tag, E Coli

Cat. Num. AAA204644

• Gene Names: PBK; SPK; CT84; TOPK; HEL164; Nori-3
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: PBK; 1-322aa; Human; His tag; E Coli; Lymphokine-activated killer T-cell-originated protein kinase; CT84; Nori-3; SPK; TOPK; PBK recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMEGISN FKTPSKLSEK KKSVLCSTPT INIPASPFMQ KLGFGTGVNV YLMKRSPRGL SHSPWAVKKI NPICNDHYRS VYQKRLMDEA KILKSLHHPN IVGYRAFTEA NDGSLCLAME YGGEKSLNDL IEERYKASQD PFPAAIILKV ALNMARGLKY LHQEKKLLHG DIKSSNVVIK GDFETIKICD VGVSLPLDEN MTVTDPEACY IGTEPWKPKE AVEENGVITD KADIFAFGLT LWEMMTLSIP HINLSNDDDD EDKTFDESDF DDEAYYAALG TRPPINMEEL DESYQKVIEL FSVCTNEDPK DRPSAAHIVE ALETDV
• Sequence Length: 322

• Applicable Applications for PBK recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PBK recombinant protein

Lymphokine-activated killer T-cell-originated protein kinase, also known as PBK, is a serine/threonine kinase related to the dual specific mitogen-activated protein kinase (MAPKK) family. PBK is abundant in placenta and absent from adult brain tissue. A PDZ domain in the tumor suppressor protein Dlg can coordinate with the T/SXV motif of PBK. This protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Recombinant human PBK protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PBK recombinant protein

Cell Cycle

References

Abe Y., et al. (2000) J Biol Chem. 275:21525-21531.; Dougherty J D., et al. (2005) J Neurosci. 25:10773-10785.;

NCBI and Uniprot Product Information

• NCBI GI #: 18490991
• NCBI GeneID: 55872
• NCBI Accession #: NP_060962
• NCBI GenBank Nucleotide #: NM_018492.3
• UniProt Accession #: Q96KB5; Q9NPD9; Q9NYL7; Q9NZK6; B4DX68; D3DST2
• Molecular Weight: 38.6 kDa (346aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
• NCBI Official Full Name: lymphokine-activated killer T-cell-originated protein kinase isoform 1
• NCBI Official Synonym Full Names: PDZ binding kinase
• NCBI Official Symbol: PBK
• NCBI Official Synonym Symbols: SPK; CT84; TOPK; HEL164; Nori-3
• NCBI Protein Information: lymphokine-activated killer T-cell-originated protein kinase
• UniProt Protein Name: Lymphokine-activated killer T-cell-originated protein kinase
• Protein Family: Lymphokine-activated killer T-cell-originated protein kinase
• UniProt Gene Name: PBK
• UniProt Synonym Gene Names: TOPK; CT84; SPK

NCBI Description

This gene encodes a serine/threonine protein kinase related to the dual specific mitogen-activated protein kinase kinase (MAPKK) family. Evidence suggests that mitotic phosphorylation is required for its catalytic activity. The encoded protein may be involved in the activation of lymphoid cells and support testicular functions, with a suggested role in the process of spermatogenesis. Overexpression of this gene has been implicated in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Uniprot Description

Phosphorylates MAP kinase p38. Seems to be active only in mitosis. May also play a role in the activation of lymphoid cells. When phosphorylated, forms a complex with TP53, leading to TP53 destabilization and attenuation of G2/M checkpoint during doxorubicin-induced DNA damage.