Mouse poly (ADP-ribose) polymerase 2 ELISA Kit | PARP2 elisa kit
Mouse Poly [ADP-ribose] polymerase 2, PARP2 ELISA Kit
Gene Names
Parp2; ARTD2; Adprt2; C78626; PARP-2; Adprtl2; Aspartl2
Reactivity
Mouse
Synonyms
poly (ADP-ribose) polymerase 2; Mouse Poly [ADP-ribose] polymerase 2; PARP2 ELISA Kit; Mouse Poly [ADP-ribose] polymerase 2 (PARP2) ELISA kit; ADPRT2; ADPRTL2; ADPRTL3; PARP-2; pADPRT-2; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) -like 2; poly (ADP-ribose) polymerase family; member 2; poly (ADP-ribosyl) transferase-like 2; poly; PARP2 elisa kit
Reactivity
Mouse
Specificity
No significant cross-reactivity or interference between Mouse PARP2 and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces and Urine
Assay Type
Sandwich
Detection Range
0.25 ng/ml - 8 ng/ml.
Sensitivity
0.1 ng/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated PARP2 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse PARP2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PARP2 and PARP2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PARP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degree C
Related Product Information for PARP2 elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated PARP2 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse PARP2 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PARP2 and PARP2 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PARP2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
63,397 Da
NCBI Official Full Name
poly
NCBI Official Synonym Full Names
poly (ADP-ribose) polymerase family, member 2
NCBI Official Symbol
Parp2
NCBI Official Synonym Symbols
ARTD2; Adprt2; C78626; PARP-2; Adprtl2; Aspartl2
NCBI Protein Information
poly [ADP-ribose] polymerase 2; poly [ADP-ribose] polymerase 2; ADPRT-2; mPARP-2; pADPRT-2; poly[ADP-ribose] synthase 2; poly[ADP-ribose] synthetase 2; NAD(+) ADP-ribosyltransferase 2; ADP-ribosyltransferase diphtheria toxin-like 2; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase) 2; ADP-ribosyltransferase (NAD+, poly(ADP-ribose) polymerase)-like 2; ADP-ribosyltransferase (NAD+; poly(ADP-ribose) polymerase)-like 2
UniProt Protein Name
Poly [ADP-ribose] polymerase 2
Protein Family
UniProt Gene Name
Parp2
UniProt Synonym Gene Names
Adprt2; Adprtl2; Aspartl2; PARP-2; mPARP-2; ARTD2; ADPRT-2; pADPRT-2
UniProt Entry Name
PARP2_MOUSE
Uniprot Description
PARP2: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transferase; DNA repair, damage; EC 2.4.2.30
Cellular Component: nucleoplasm; nucleolus; nucleus
Molecular Function: transferase activity; transferase activity, transferring glycosyl groups; protein binding; DNA binding; NAD+ ADP-ribosyltransferase activity
Biological Process: protein amino acid ADP-ribosylation; base-excision repair; DNA repair
Research Articles on PARP2
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Product Notes
The Mouse PARP2 parp2 (Catalog #AAA9320473) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9320473 ELISA Kit recognizes Mouse PARP2. It is sometimes possible for the material contained within the vial of "poly (ADP-ribose) polymerase 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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