Poly (A)-specific ribonuclease PARN (Parn) Recombinant Protein | Parn recombinant protein

Recombinant Mouse Poly (A)-specific ribonuclease PARN (Parn)

Cat. Num. AAA1286453

• Gene Names: Parn; DAN; 1200003I18Rik
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Poly (A)-specific ribonuclease PARN (Parn); Recombinant Mouse Poly (A)-specific ribonuclease PARN (Parn); Parn recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 1-624, Full length protein
• Sequence: MEIIRSNFKINLHKVYQAIEEADFFAIDGEFSGISDGPSVTALTSGFDTPEERYQKLKKHSMDFLLFQFGLCAFKYDHTDSKHVTKSFNFYVFPKPFSRSSPDVKFVCQSSSIDFLASQGFDFNKVFCSGIPYLNQEEERQLREQFDEKRSQANGAGALAKCPVTIPEDQKKFIDQVIEKIEDFLQSEEKRSLELDPCTGFQRKLIYQTLSWKYPKGIHVETLETDKKERHIVISKVDEEERKRREQEKYTKEQEELNDAVGFSRVIHAIANSGKLVVGHNMLLDVMHTIHQFYCPLPADLNEFKEMAICVFPRLLDTKLMASTQPFKDIINNTSLAELEKRLKETPFDPPKVESAEGFPSYDTASEQLHEAGYDAYITGLCFISMANYLGSLLSPPKMCVSARSKLIEPFFNKLFLMRVMDIPYLNLEGPDLQPKRDHVLHVTFPKEWKTSDLYQLFSAFGNIQISWIDDTSAFVSLSQPEQVQIAVNTSKYAESYRIQTYAEYVGKKQEGKQVKRKWTEDSWKEVDRKRPHMQGPCYHSNSFTAAGVLGKRTLSPDPREAALEDRESEEVSDSELEQTDSCTDPLPEGRKKSKKLKRMKKELSLAGSVSDSPAVLFEVPDTW
• Sequence Length: 624

• Species: Mouse
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for Parn recombinant protein

This protein is a 3 -exoribonuclease, with similarity to the RNase D family of 3 -exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

NCBI and Uniprot Product Information

• NCBI GI #: 21311877
• NCBI GeneID: 74108
• NCBI Accession #: NP_083037.1
• NCBI GenBank Nucleotide #: NM_028761.3
• UniProt Accession #: Q8VDG3; Q8C7N6; Q9DC46
• Molecular Weight: 71,559 Da
• NCBI Official Full Name: poly(A)-specific ribonuclease PARN isoform 1
• NCBI Official Synonym Full Names: poly(A)-specific ribonuclease (deadenylation nuclease)
• NCBI Official Symbol: Parn
• NCBI Official Synonym Symbols: DAN; 1200003I18Rik
• NCBI Protein Information: poly(A)-specific ribonuclease PARN
• UniProt Protein Name: Poly(A)-specific ribonuclease PARN
• Protein Family: Poly(A)-specific ribonuclease
• UniProt Gene Name: Parn

Uniprot Description

3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization ().