Human L-Phenylalanine Ammonia-Lyase ELISA Kit | PAL elisa kit

Human L-Phenylalanine Ammonia-Lyase ELISA Kit

Cat. Num. AAA30500

• Reactivity: Human
• Synonyms: L-Phenylalanine Ammonia-Lyase; Human L-Phenylalanine Ammonia-Lyase ELISA Kit; PAL elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human PAL and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 1.56 U/L - 50 U/L.
• Sensitivity: 0.1 U/L.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated PAL concentrations in Human serum, plasma and other body fluids. Using Purified Human PAL antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PAL and PAL antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PAL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for PAL elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated PAL concentrations in Human serum, plasma and other body fluids. Using Purified Human PAL antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add PAL and PAL antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of PAL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 288351
• UniProt Accession #: P11544
• Molecular Weight: 76,880 Da
• NCBI Official Full Name: L-phenylalanine ammonia-lyase
• UniProt Protein Name: Phenylalanine/tyrosine ammonia-lyase
• Protein Family: Lysin
• UniProt Gene Name: PAL
• UniProt Synonym Gene Names: Bifunctional PAL
• UniProt Entry Name: PALY_RHOTO

Uniprot Description

Function: Catalyzes the non-oxidative deamination of L-phenylalanine and L-tyrosine to form trans-cinnamic acid and p-coumaric acid respectively with similar efficiencies. Facilitates the commitment step in phenylpropanoid pathways that produce lignins, coumarins and flavonoids. Ref.4

Catalytic activity: L-phenylalanine = trans-cinnamate + ammonia. Ref.4L-tyrosine = trans-p-hydroxycinnamate + ammonia. Ref.4

Pathway: Phenylpropanoid metabolism; trans-cinnamate biosynthesis; trans-cinnamate from L-phenylalanine: step 1/1.

Subunit structure: Homodimer. Ref.5

Subcellular location: Cytoplasm

Probable.

Post-translational modification: Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly.

Sequence similarities: Belongs to the PAL/histidase family.

Biophysicochemical propertiesKinetic parameters:KM=0.29 mM for L-phenylalanine Ref.4 Ref.5KM=0.18 mM for L-tyrosinepH dependence:Optimum pH is 8.5.