PAK3 recombinant protein

Recombinant Human PAK3 Protein (His tag)

Cat. Num. AAA2545066

• Gene Names: PAK3; ARA; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3beta; beta-PAK
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: PAK3; Recombinant Human PAK3 Protein (His tag); RP5-914P14.3; CDKN1A; MRX30; MRX47; OPHN3; PAK3beta; bPAK; Hpak3; PAK3 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% glycerol
• Sequence Length: 544

• Application Notes: The recombinant human PAK3 consists of 554 amino acids and predicts a molecular mass of 62 kDa. It migrates as an approximately 60 kDa band in SDS-PAGE in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met 1
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

Related Product Information for PAK3 recombinant protein

Background: PAK3 is a member of PAK proteins, a family of serine/threonine p21-activating kinases, serve as effectors of small Rho GTPases Cdc42 and RAC and have been implicated in a wide range of biological activities. There are six mammalian PAKs which can be divided into two groups: group I PAKs (PAK1-3) and group II PAKs (PAK4-6). Although the two PAK groups are architecturally similar there are differences in their mode of regulation suggesting their cellular functions are likely to be different. Group I p21-activated kinases (PAK1/2/3) is demonstrated as ERK3/ERK4 activation loop kinases. It has been shown that group I PAKs phosphorylate ERK3 and ERK4 on Ser-189 and Ser-186, respectively, both in vitro and in vivo, and that expression of activated Rac1 augments this response. Besides regulation enzymatic activation of ERK3/ERK4, PAKs can also play roles in downstream activation of MAP kinase-activated protein kinase 5 (MK5) in vivo. Thus, the group I PAKs act as upstream activators of ERK3 and ERK4 and unravel a novel PAK-ERK3/ERK4-MK5 signaling pathway. In clinical, PAK has been proposed as a potential therapeutic target in schwannomas.

Description: A DNA sequence encoding the human PAK3 isoform 2 (O75914-2) (Met 1-Arg 544) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 189491751
• NCBI GeneID: 5063
• NCBI Accession #: NP_001121638.1
• NCBI GenBank Nucleotide #: NM_001128166.2
• UniProt Accession #: O75914; Q5JWX1; Q5JWX2; Q7Z2D6; Q7Z2E4; Q7Z3Z8; Q8WWK5; Q8WX23; A8K389; B1GX77; B1GX78; B1GX79
• Molecular Weight: 62,913 Da
• NCBI Official Full Name: serine/threonine-protein kinase PAK 3 isoform a
• NCBI Official Synonym Full Names: p21 (RAC1) activated kinase 3
• NCBI Official Symbol: PAK3
• NCBI Official Synonym Symbols: ARA; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3beta; beta-PAK
• NCBI Protein Information: serine/threonine-protein kinase PAK 3
• UniProt Protein Name: Serine/threonine-protein kinase PAK 3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PAK3
• UniProt Synonym Gene Names: OPHN3; PAK-3

NCBI Description

The protein encoded by this gene is a serine-threonine kinase and forms an activated complex with GTP-bound RAS-like (P21), CDC2 and RAC1. This protein may be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Defects in this gene are the cause of non-syndromic mental retardation X-linked type 30 (MRX30), also called X-linked mental retardation type 47 (MRX47). Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Apr 2016]

Uniprot Description

PAK3: a protein kinase of the STE20 family that regulates synapse formation and plasticity in the hippocampus. May be necessary for dendritic development and for the rapid cytoskeletal reorganization in dendritic spines associated with synaptic plasticity. Forms an activated complex with GTP-bound P21, CDC2 and RAC1 proteins. Missense and truncation mutations linked to nonsyndromic mental retardation type 30 (MRX30). Two alternatively spliced human isoforms have been reported.

Protein type: EC 2.7.11.1; Kinase, protein; PAKA subfamily; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family

Chromosomal Location of Human Ortholog: Xq23

Cellular Component: cytoplasm; cytosol; plasma membrane

Molecular Function: MAP kinase kinase activity; protein binding; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: axonogenesis; dendrite development; ephrin receptor signaling pathway; mitotic cell cycle; regulation of actin filament polymerization; stimulatory C-type lectin receptor signaling pathway; synapse organization and biogenesis; T cell costimulation; T cell receptor signaling pathway

Disease: Mental Retardation, X-linked 30