Rat Integrin Alpha M ELISA Kit | ITGalphaM elisa kit

Rat Integrin Alpha M ELISA Kit

Cat. Num. AAA35901

• Gene Names: ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
• Reactivity: Rat
• Synonyms: Integrin Alpha M; N/A; Rat Integrin Alpha M ELISA Kit; ITGalphaM elisa kit

• Reactivity: Rat
• Specificity: No significant cross-reactivity or interference between Rat ITGalphaM and analogues was observed.
• Sequence Length: 1153
• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 3.12 ng/ml - 100 ng/ml.
• Sensitivity: 1.0 ng/ml.

• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated ITGalphaM concentrations in Rat serum, plasma and other body fluids. Using Purified Rat ITGalphaM antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ITGalphaM and ITGalphaM antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ITGalphaM in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for ITGalphaM elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated ITGalphaM concentrations in Rat serum, plasma and other body fluids. Using Purified Rat ITGalphaM antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ITGalphaM and ITGalphaM antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ITGalphaM in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 224831239
• NCBI GeneID: 3684
• NCBI Accession #: NP_001139280.1
• NCBI GenBank Nucleotide #: NM_001145808.1
• UniProt Accession #: P11215; Q4VAK0; Q4VAK1; Q4VAK2
• Molecular Weight: 127,179 Da
• NCBI Official Full Name: integrin alpha-M isoform 1
• NCBI Official Synonym Full Names: integrin, alpha M (complement component 3 receptor 3 subunit)
• NCBI Official Symbol: ITGAM
• NCBI Official Synonym Symbols: CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
• NCBI Protein Information: integrin alpha-M; CR-3 alpha chain; antigen CD11b (p170); leukocyte adhesion receptor MO1; CD11 antigen-like family member B; macrophage antigen alpha polypeptide; cell surface glycoprotein MAC-1 subunit alpha; neutrophil adherence receptor alpha-M subunit
• UniProt Protein Name: Integrin alpha-M
• UniProt Gene Name: ITGAM
• UniProt Synonym Gene Names: CD11B; CR3A
• UniProt Entry Name: ITAM_HUMAN