Pup--protein ligase (pafA) Recombinant Protein | pafA recombinant protein
Recombinant Streptosporangium roseum Pup--protein ligase (pafA)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Pup--protein ligase (pafA); Recombinant Streptosporangium roseum Pup--protein ligase (pafA); pafA recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-452, Full length protein
Sequence
MDRRIFGLENEYGVTCTFRGQRRLSPDEVARYLFRRVVSWGRSSNVFLRNGARLYLDVGSHPEYATPECDNVVELVTHDKAGERILEGLLVDAEKRLREEGIAGDIYLFKNNTDSAGNSYGCHENYLVGRHGEFGRLADVLIPFLVTRQIVCGAGKVLQTPRGAVYCVSQRAEHIWEGVSSATTRSRPIINTRDEPHADAERFRRLHVIVGDSNMSETTMLLKVGATDLVLRMIEAGTVMRDLSLENPIRAIREVSHDMTGRRRVRLANGREASSLEIQQEYLSKARDFVDRRGGDEISHRVLELWERTLNAVETGNLDLVAREIDWVTKYQLIERYRKKYDLPLSSPRVAQLDLAYHDVHRRRGLFYLLQKRGAVERVASDLKIFEAKSVPPQTTRARLRGEFIRKAQEKRRDFTVDWVHLKLNDQAQRTVLCKDPFRSVDERVDKLIAGM
Sequence Length
452
Species
Streptosporangium roseum (strain ATCC 12428 / DSM 43021 / JCM 3005 / NI 9100)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
51,890 Da
NCBI Official Full Name
Pup--protein ligase
UniProt Protein Name
Pup--protein ligase
Protein Family
UniProt Gene Name
pafA
Uniprot Description
Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine.
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Product Notes
The pafA pafa (Catalog #AAA1178925) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-452, Full length protein. The amino acid sequence is listed below: MDRRIFGLEN EYGVTCTFRG QRRLSPDEVA RYLFRRVVSW GRSSNVFLRN GARLYLDVGS HPEYATPECD NVVELVTHDK AGERILEGLL VDAEKRLREE GIAGDIYLFK NNTDSAGNSY GCHENYLVGR HGEFGRLADV LIPFLVTRQI VCGAGKVLQT PRGAVYCVSQ RAEHIWEGVS SATTRSRPII NTRDEPHADA ERFRRLHVIV GDSNMSETTM LLKVGATDLV LRMIEAGTVM RDLSLENPIR AIREVSHDMT GRRRVRLANG REASSLEIQQ EYLSKARDFV DRRGGDEISH RVLELWERTL NAVETGNLDL VAREIDWVTK YQLIERYRKK YDLPLSSPRV AQLDLAYHDV HRRRGLFYLL QKRGAVERVA SDLKIFEAKS VPPQTTRARL RGEFIRKAQE KRRDFTVDWV HLKLNDQAQR TVLCKDPFRS VDERVDKLIA GM. It is sometimes possible for the material contained within the vial of "Pup--protein ligase (pafA), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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