Rat Procollagen Type III N-Terminal Propeptide ELISA Kit | P3NP elisa kit

Rat Procollagen Type III N-Terminal Propeptide ELISA Kit

Cat. Num. AAA12964

• Reactivity: Rat
• Synonyms: Procollagen Type III N-Terminal Propeptide; Rat Procollagen Type III N-Terminal Propeptide ELISA Kit; P3NP elisa kit

• Reactivity: Rat
• Specificity: No significant cross-reactivity or interference between Rat P3NP and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces and Urine
• Assay Type: Sandwich
• Detection Range: 3.12ng/ml-100ng/ml.
• Sensitivity: 1.0ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated P3NP concentrations in Rat serum, plasma and other body fluids. Using Purified Rat P3NP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add P3NP and P3NP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of P3NP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for P3NP elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated P3NP concentrations in Rat serum, plasma and other body fluids. Using Purified Rat P3NP antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add P3NP and P3NP antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of P3NP in the samples is then determined by comparing the O.D. of the samples to the standard curve.