Rat Phosphorylated tau 231 ELISA Kit | p-Tau231 elisa kit
Rat Phosphorylated tau 231 ELISA Kit
Reactivity
Rat
Synonyms
Phosphorylated tau 231; Rat Phosphorylated tau 231 ELISA Kit; p-Tau231 elisa kit
Reactivity
Rat
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Sensitivity
0.1 ng/ml.
Intended Uses
This p-Tau231 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat p-Tau231. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for p-Tau231 elisa kit
Principle of the assay: p-Tau231 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-p-Tau231 antibody and an p-Tau231-HRP conjugate. The assay sample and buffer are incubated together with p-Tau231-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the p-Tau231 concentration since p-Tau231 from samples and p-Tau231-HRP conjugate compete for the anti-p-Tau231 antibody binding site. Since the number of sites is limited, as more sites are occupied by p-Tau231 from the sample, fewer sites are left to bind p-Tau231-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The p-Tau231 concentration in each sample is interpolated from this standard curve.
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