Canine Oxidized Low Density Lipoprotein ELISA Kit | OxLDL elisa kit

Canine Oxidized Low Density Lipoprotein (OxLDL) ELISA Kit

Cat. Num. AAA2606949

• Gene Names: OLR1; LOX1; LOXIN; SLOX1; CLEC8A; SCARE1
• Reactivity: Canine
• Synonyms: Oxidized Low Density Lipoprotein; Canine Oxidized Low Density Lipoprotein (OxLDL) ELISA Kit; OxLDL elisa kit

• Reactivity: Canine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 273

• Samples: Canine Serum, Plasma Or Cell Culture Supernatant And Organizations In The Natural And Recombinant Ot Concentration
• Assay Type: Sandwich
• Detection Range: 1000 pg/ml-15.6 pg/ml
• Sensitivity: 5 pg/ml.
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OxLDL elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Canine OT monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 18490153
• NCBI GeneID: 4973
• Molecular Weight: 21,425 Da
• NCBI Official Full Name: Oxidized low density lipoprotein (lectin-like) receptor 1
• NCBI Official Synonym Full Names: oxidized low density lipoprotein receptor 1
• NCBI Official Symbol: OLR1
• NCBI Official Synonym Symbols: LOX1; LOXIN; SLOX1; CLEC8A; SCARE1
• NCBI Protein Information: oxidized low-density lipoprotein receptor 1
• UniProt Protein Name: Oxidized low-density lipoprotein receptor 1
• UniProt Gene Name: OLR1
• UniProt Synonym Gene Names: CLEC8A; LOX1; Ox-LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; hLOX-1
• UniProt Entry Name: OLR1_HUMAN

NCBI Description

This gene encodes a low density lipoprotein receptor that belongs to the C-type lectin superfamily. This gene is regulated through the cyclic AMP signaling pathway. The encoded protein binds, internalizes and degrades oxidized low-density lipoprotein. This protein may be involved in the regulation of Fas-induced apoptosis. This protein may play a role as a scavenger receptor. Mutations of this gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

Uniprot Description

OLR1: Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro- oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro- atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram- positive bacteria. Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility. OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 12p13.2-p12.3

Cellular Component: integral to plasma membrane; intracellular membrane-bound organelle; lipid raft; membrane; nucleoplasm; plasma membrane; receptor complex

Molecular Function: carbohydrate binding; low-density lipoprotein receptor activity; protein binding

Biological Process: blood circulation; blood coagulation; cell death; inflammatory response; leukocyte adhesion; leukocyte migration; lipoprotein metabolic process; proteolysis; receptor-mediated endocytosis; response to hydrogen peroxide

Disease: Myocardial Infarction, Susceptibility To