Bovine Oxidized Lowdensity Lipoprotein ELISA Kit | OXLDL elisa kit

Bovine Oxidized Lowdensity Lipoprotein ELISA Kit

Cat. Num. AAA12479

• Gene Names: OLR1; LOX1; LOXIN; SLOX1; CLEC8A; SCARE1
• Reactivity: Bovine
• Synonyms: Oxidized Lowdensity Lipoprotein; Bovine Oxidized Lowdensity Lipoprotein ELISA Kit; OXLDL elisa kit

• Reactivity: Bovine
• Specificity: No significant cross-reactivity or interference between Bovine OXLDL and analogues was observed.
• Sequence Length: 273

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 31.2 ng/ml – 1000 ng/ml.
• Sensitivity: 5.0 ng/ml.
• Intended Uses: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate OXLDL concentrations in Bovine serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic applications
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OXLDL elisa kit

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate OXLDL concentrations in Bovine serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research!

NCBI and Uniprot Product Information

• NCBI GI #: 4505501
• NCBI GeneID: 4973
• NCBI Accession #: NP_002534.1
• NCBI GenBank Nucleotide #: NM_002543.3
• UniProt Accession #: P78380; Q2PP00; Q7Z484; A8K7V9; B4DI48; G3V1I4
• Molecular Weight: 30,959 Da
• NCBI Official Full Name: oxidized low-density lipoprotein receptor 1 isoform 1
• NCBI Official Synonym Full Names: oxidized low density lipoprotein (lectin-like) receptor 1
• NCBI Official Symbol: OLR1
• NCBI Official Synonym Symbols: LOX1; LOXIN; SLOX1; CLEC8A; SCARE1
• NCBI Protein Information: oxidized low-density lipoprotein receptor 1; hLOX-1; ox LDL receptor 1; lectin-type oxidized LDL receptor 1; scavenger receptor class E, member 1; C-type lectin domain family 8 member A; oxidized low-density lipoprotein receptor 1, soluble form
• UniProt Protein Name: Oxidized low-density lipoprotein receptor 1
• UniProt Gene Name: OLR1
• UniProt Synonym Gene Names: CLEC8A; LOX1; Ox-LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; hLOX-1
• UniProt Entry Name: OLR1_HUMAN

NCBI Description

This gene encodes a low density lipoprotein receptor that belongs to the C-type lectin superfamily. This gene is regulated through the cyclic AMP signaling pathway. The encoded protein binds, internalizes and degrades oxidized low-density lipoprotein. This protein may be involved in the regulation of Fas-induced apoptosis. This protein may play a role as a scavenger receptor. Mutations of this gene have been associated with atherosclerosis, risk of myocardial infarction, and may modify the risk of Alzheimer's disease. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

Uniprot Description

OLR1: Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro- oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro- atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram- positive bacteria. Independent association genetic studies have implicated OLR1 gene variants in myocardial infarction susceptibility. OLR1 may be involved in Alzheimer disease (AD). Involvement in AD is however unclear: according to some authors (PubMed:12354387, PubMed:12810610 and PubMed:15976314), variations in OLR1 modify the risk of AD, while according to other (PubMed:15000751 and PubMed:15060104) they do not. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 12p13.2-p12.3

Cellular Component: nucleoplasm; membrane; intracellular membrane-bound organelle; integral to plasma membrane; plasma membrane; receptor complex; lipid raft

Molecular Function: low-density lipoprotein receptor activity; protein binding; carbohydrate binding

Biological Process: cell death; receptor-mediated endocytosis; leukocyte adhesion; response to hydrogen peroxide; blood circulation; lipoprotein metabolic process; blood coagulation; proteolysis; inflammatory response; leukocyte migration

Disease: Myocardial Infarction, Susceptibility To