Osteocrin Recombinant Protein | OSTN recombinant protein
Osteocrin, Recombinant, Human
Gene Names
OSTN; MUSCLIN
Purity
Highly Purified95% (SDS-PAGE); One-step procedure using affinity Ni-NTA chromatography.
Synonyms
Osteocrin; Recombinant; Human; OSTN recombinant protein
Host
E Coli
Specificity
The amino acid sequence of the recombinant human Osteocrin is 100% homologous to the amino acid sequence of the human Osteocrin without signal sequence.
Purity/Purification
Highly Purified
95% (SDS-PAGE); One-step procedure using affinity Ni-NTA chromatography.
95% (SDS-PAGE); One-step procedure using affinity Ni-NTA chromatography.
Form/Format
Supplied as a lyophilized powder in 5mM Tris, 25mM sodium chloride, pH 7.5.
Application Notes
Western blotting, ELISA
Reconstitution
Add 0.2 ml of dH2O and let the lyophilized pellet dissolve completely. Storage: Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.
Preparation and Storage
-20 degree C
Related Product Information for OSTN recombinant protein
Osteocrin is a recently identified secreted protein expression of which was only detected in bone, peaking just after birth and decreasing markedly with age. A 1280-bp mRNA encodes osteocrin producing a mature protein of 103 amino acids with a molecular mass of 11.4kD. In primary osteoblastic cell cultures osteocrin expression coincided with matrix formation then decreased in very mature cultures. Treatment of cultures with 1,25-dihydroxyvitamin D3 resulted in a rapid dosedependent down-regulation of osteocrin expression, suggesting direct regulation. Chronic treatment of primary cultures with osteocrin-conditioned media inhibited mineralization and reduced osteocalcin and alkaline phosphatase expression. These results suggest that osteocrin represents a novel, unique vitamin D-regulated bone-specific protein that appears to act as a soluble osteoblast regulator.
The recombinant Human Osteocrin is produced with N-terminal fusion of HisTag. The Human Osteocrin His-Tagged Fusion Protein is 13.6kD containing 106 amino acid residues of the human Osteocrin and 16 additional amino acid residues - HisTag (bold). MRGSHHHHHH GMASHMVDVT TTEAFDSGVI DVQSTPTVRE EKSATDLTAK LLLLDELVSL ENDVIETKKK RSFSGFGSPL DRLSAGSVDH KGKQRKVVDH PKRRFGIPMD RIGRNRLSNS RG
The recombinant Human Osteocrin is produced with N-terminal fusion of HisTag. The Human Osteocrin His-Tagged Fusion Protein is 13.6kD containing 106 amino acid residues of the human Osteocrin and 16 additional amino acid residues - HisTag (bold). MRGSHHHHHH GMASHMVDVT TTEAFDSGVI DVQSTPTVRE EKSATDLTAK LLLLDELVSL ENDVIETKKK RSFSGFGSPL DRLSAGSVDH KGKQRKVVDH PKRRFGIPMD RIGRNRLSNS RG
Product Categories/Family for OSTN recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13.6kD
NCBI Official Full Name
osteocrin
NCBI Official Synonym Full Names
osteocrin
NCBI Official Symbol
OSTN
NCBI Official Synonym Symbols
MUSCLIN
NCBI Protein Information
osteocrin
UniProt Protein Name
Osteocrin
Protein Family
UniProt Gene Name
OSTN
UniProt Entry Name
OSTN_HUMAN
Uniprot Description
OSTN: Appears to modulate osteoblastic differentiation. Could also function as an autocrine and paracrine factor linked to glucose metabolism in skeletal muscle.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 3q28
Cellular Component: extracellular space
Molecular Function: hormone activity
Biological Process: negative regulation of glucose import; ossification; cell surface receptor linked signal transduction; positive regulation of cGMP biosynthetic process; negative regulation of osteoblast differentiation; cell differentiation
Research Articles on OSTN
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Product Notes
The OSTN ostn (Catalog #AAA637978) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. Western blotting, ELISA. Researchers should empirically determine the suitability of the OSTN ostn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Osteocrin, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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