Outer Surface Protein A Recombinant Protein | ospA recombinant protein
Recombinant Borrelia Afzelii Outer Surface Protein A
Applications
Western Blot
Purity
Greater than 80.0% as determined by SDS-PAGE.
Synonyms
Outer Surface Protein A; Recombinant Borrelia Afzelii Outer Surface Protein A; Borrelia Afzelii OspA; Borrelia Afzelii Outer Surface Protein A Recombinant; Borrelia OspA; ospA recombinant protein
Host
E Coli
Purity/Purification
Greater than 80.0% as determined by SDS-PAGE.
Form/Format
Borrelia Afzelii OspA is supplied in 20mM HEPES buffer pH-7.6, 250mM NaCl and 20% glycerol.
Sterile Filtered clear solution.
Sterile Filtered clear solution.
Sequence Length
273
Applicable Applications for ospA recombinant protein
Western Blot (WB)
Application Notes
Western blot with Lyme positive plasma.
Immunological Functions
1. Binds IgG- and IgM-type human antibodies.2. Immunodot test with Lyme disease positive/negative plasma; suitable for LTT (lymphocyte transformation test).
Preparation and Storage
Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. Avoid multiple freeze-thaw cycles.
Related Product Information for ospA recombinant protein
Description: Recombinant Borrelia Afzelii Outer Surface Protein A produced in E Coli is a non-glycosylated, polypeptide chain having a calculated molecular mass of 29,074 Dalton. Borrelia Afzelii OspA is expressed with a -6x His tag at N-terminus and purified by proprietary chromatographic techniques.
Introduction: Borrelia belongs to a genus of bacteria of the spirochete phylum. Borrelia causes borreliosis, which is a zoonotic, vector-borne disease transmitted mainly by ticks and some by lice, depending on the species. Of the 36 known species of Borrelia, 12 are distinguished to cause Lyme disease or borreliosis and are transmitted by ticks. The main Borrelia species causing Lyme disease are Borrelia burgdorferi, Borrelia afzelii, and Borrelia garinii. The Borrelia genus members have a linear chromosome which is about 900 kbp in length as well as an excess of both linear and circular plasmids in the 5-220 kbp size range. The plasmids are atypical, as compared to most bacterial plasmids, since they contain many paralogous sequences, a large number of pseudogenes and, in some cases, essential genes. Moreover, a number of the plasmids have features suggesting that they are prophages.
Introduction: Borrelia belongs to a genus of bacteria of the spirochete phylum. Borrelia causes borreliosis, which is a zoonotic, vector-borne disease transmitted mainly by ticks and some by lice, depending on the species. Of the 36 known species of Borrelia, 12 are distinguished to cause Lyme disease or borreliosis and are transmitted by ticks. The main Borrelia species causing Lyme disease are Borrelia burgdorferi, Borrelia afzelii, and Borrelia garinii. The Borrelia genus members have a linear chromosome which is about 900 kbp in length as well as an excess of both linear and circular plasmids in the 5-220 kbp size range. The plasmids are atypical, as compared to most bacterial plasmids, since they contain many paralogous sequences, a large number of pseudogenes and, in some cases, essential genes. Moreover, a number of the plasmids have features suggesting that they are prophages.
Product Categories/Family for ospA recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
29,640 Da
NCBI Official Full Name
outer surface protein A
UniProt Protein Name
Outer surface protein A
Protein Family
UniProt Gene Name
ospA
UniProt Entry Name
OSPA5_BORBG
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Product Notes
The Outer Surface Protein An ospa (Catalog #AAA145103) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Outer Surface Protein A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western blot with Lyme positive plasma. Researchers should empirically determine the suitability of the Outer Surface Protein An ospa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Outer Surface Protein A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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