Sheep Osteoclast Stimulating Factor 1 ELISA Kit | OSF1 elisa kit

Sheep Osteoclast Stimulating Factor 1 ELISA Kit

Cat. Num. AAA734323

• Gene Names: OSTF1; OSF; SH3P2; bA235O14.1
• Reactivity: Sheep
• Synonyms: Osteoclast Stimulating Factor 1; Sheep Osteoclast Stimulating Factor 1 ELISA Kit; OSF1 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This OSF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep OSF. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OSF1 elisa kit

Principle of the Assay: OSF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-OSF antibody and an OSF-HRP conjugate. The assay sample and buffer are incubated together with OSF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OSF concentration since OSF from samples and OSF-HRP conjugate compete for the anti-OSF antibody binding site. Since the number of sites is limited, as more sites are occupied by OSF from the sample, fewer sites are left to bind OSF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OSF concentration in each sample is interpolated from this standard curve.

Product Categories/Family for OSF1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 166235148
• NCBI GeneID: 26578
• NCBI Accession #: NP_036515.4
• NCBI GenBank Nucleotide #: NM_012383.4
• Molecular Weight: 23,787 Da
• NCBI Official Full Name: osteoclast-stimulating factor 1
• NCBI Official Synonym Full Names: osteoclast stimulating factor 1
• NCBI Official Symbol: OSTF1
• NCBI Official Synonym Symbols: OSF; SH3P2; bA235O14.1
• NCBI Protein Information: osteoclast-stimulating factor 1
• UniProt Protein Name: Osteoclast-stimulating factor 1
• UniProt Gene Name: OSTF1
• UniProt Entry Name: OSTF1_HUMAN

NCBI Description

Osteoclast-stimulating factor-1 is an intracellular protein produced by osteoclasts that indirectly induces osteoclast formation and bone resorption (Reddy et al., 1998 [PubMed 10092216]).[supplied by OMIM, Mar 2008]

Uniprot Description

OSTF1: Induces bone resorption, acting probably through a signaling cascade which results in the secretion of factor(s) enhancing osteoclast formation and activity.

Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 9q13-q21.2

Cellular Component: cytoplasm; intracellular

Molecular Function: protein binding; SH3 domain binding

Biological Process: ossification; signal transduction