Human osteoclast associated, immunoglobulin-like receptor ELISA Kit | OSCAR elisa kit

Human Osteoclast-associated immunoglobulin-like receptor, OSCAR ELISA Kit

Cat. Num. AAA946901

• Gene Names: OSCAR; PIGR3; PIgR-3
• Reactivity: Human
• Synonyms: osteoclast associated; immunoglobulin-like receptor; Human Osteoclast-associated immunoglobulin-like receptor; OSCAR ELISA Kit; Human Osteoclast-associated immunoglobulin-like receptor (OSCAR) ELISA kit; MGC33613; PIGR3; osteoclast associated receptor OSCAR-S1; osteoclast associated receptor OSCAR-S2; osteoclast-associated receptor; polymeric immunoglobulin receptor 3; OSCAR elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human OSCAR. No significant cross-reactivity or interference between human OSCAR and analogues was observed.
• Sequence Length: 282

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 6.25 ng/ml - 400 ng/ml.
• Sensitivity: The minimum detectable dose of human OSCAR is typically less than 1.56 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for OSCAR elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for OSCAR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any OSCAR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for OSCAR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of OSCAR bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 537544647
• NCBI GeneID: 126014
• NCBI Accession #: NP_001269278.1
• NCBI GenBank Nucleotide #: NM_001282349.1
• UniProt Accession #: Q8IYS5; Q5GRG5; Q8N763; Q8NHL4; Q8WXQ0; Q8WXQ1; Q8WXQ2; B7WNS2
• Molecular Weight: 30,481 Da
• NCBI Official Full Name: osteoclast-associated immunoglobulin-like receptor isoform 6
• NCBI Official Synonym Full Names: osteoclast associated, immunoglobulin-like receptor
• NCBI Official Symbol: OSCAR
• NCBI Official Synonym Symbols: PIGR3; PIgR-3
• NCBI Protein Information: osteoclast-associated immunoglobulin-like receptor; poly-Ig receptor 3; polymeric immunoglobulin receptor 3; osteoclast associated receptor OSCAR-S1; osteoclast associated receptor OSCAR-S2
• UniProt Protein Name: Osteoclast-associated immunoglobulin-like receptor
• Protein Family: Osteoclast-associated immunoglobulin-like receptor
• UniProt Gene Name: OSCAR
• UniProt Synonym Gene Names: Osteoclast-associated receptor; hOSCAR; PIgR-3; PIgR3; Poly-Ig receptor 3
• UniProt Entry Name: OSCAR_HUMAN

NCBI Description

Osteoclasts are multinucleated cells that resorb bone and are essential for bone homeostasis. This gene encodes an osteoclast-associated receptor (OSCAR), which is a member of the leukocyte receptor complex protein family that plays critical roles in the regulation of both innate and adaptive immune responses. The encoded protein may play a role in oxidative stress-mediated atherogenesis as well as monocyte adhesion. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2013]

Uniprot Description

OSCAR: Regulator of osteoclastogenesis which plays an important bone-specific function in osteoclast differentiation. Belongs to the leukocyte receptor complex/polymeric immunogobulin receptor (PIR/LRC) family. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19q13.42

Cellular Component: plasma membrane; integral to membrane