Enzymatic polyprotein (ORF V) Recombinant Protein | ORF V recombinant protein

Recombinant Cestrum yellow leaf curling virus Enzymatic polyprotein (ORF V), partial

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Enzymatic polyprotein (ORF V); Recombinant Cestrum yellow leaf curling virus Enzymatic polyprotein (ORF V); partial; ORF V recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 643

• Species: Cestrum yellow leaf curling virus (CmYLCV)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 32306489
• NCBI GeneID: 1732955
• NCBI Accession #: NP_861410.1
• NCBI GenBank Nucleotide #: NC_004324.3
• UniProt Accession #: Q7TD08
• Molecular Weight: 74,082 Da
• NCBI Official Full Name: putative multifunctional pol protein
• NCBI Official Symbol: CmYLCVgp4
• NCBI Protein Information: ORF V
• UniProt Protein Name: Enzymatic polyprotein
• UniProt Gene Name: ORF V

Uniprot Description

Encodes for at least two polypeptides: protease (PR) and reverse transcriptase (RT). The protease processes the polyprotein in cis. Reverse transcriptase is multifunctional enzyme that converts the viral RNA genome into dsDNA in viral cytoplasmic capsids. This enzyme displays a DNA polymerase activity that can copy either DNA or RNA templates, and a ribonuclease H (RNase H) activity that cleaves the RNA strand of RNA-DNA heteroduplexes in a partially processive 3'- to 5'-endonucleasic mode. Neo-synthesized pregenomic RNA (pgRNA) are encapsidated, and reverse-transcribed inside the nucleocapsid. Partial (+)DNA is synthesized from the (-)DNA template and generates the relaxed circular DNA (RC-DNA) genome. After budding and infection, the RC-DNA migrates in the nucleus, and is converted into a plasmid-like covalently closed circular DNA (cccDNA) ().