Cat Osteonectin ELISA Kit | ON elisa kit

Cat Osteonectin ELISA Kit

• Gene Names: SPARC; ON
• Reactivity: Cat
• Synonyms: Osteonectin; Cat Osteonectin ELISA Kit; ON elisa kit

• Reactivity: Cat
• Specificity: No significant cross-reactivity or interference between Cat ON and analogues was observed.

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 3.12ng/ml-100ng/ml.
• Sensitivity: 1.0ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated ON concentrations in Cat serum, plasma and other body fluids. Using Purified Cat ON antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ON and ON antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ON in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) are less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for ON elisa kit

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated ON concentrations in Cat serum, plasma and other body fluids. Using Purified Cat ON antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add ON and ON antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ON in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 338325
• NCBI GeneID: 6678
• UniProt Accession #: P09486; Q6IBK4; D3DQH9
• Molecular Weight: 34,632 Da
• NCBI Official Full Name: osteonectin
• NCBI Official Synonym Full Names: secreted protein, acidic, cysteine-rich (osteonectin)
• NCBI Official Symbol: SPARC
• NCBI Official Synonym Symbols: ON
• NCBI Protein Information: SPARC; BM-40; osteonectin; cysteine-rich protein; basement-membrane protein 40; secreted protein acidic and rich in cysteine
• UniProt Protein Name: SPARC
• UniProt Gene Name: SPARC
• UniProt Synonym Gene Names: ON; BM-40; ON
• UniProt Entry Name: SPRC_HUMAN

NCBI Description

This gene encodes a cysteine-rich acidic matrix-associated protein. The encoded protein is required for the collagen in bone to become calcified but is also involved in extracellular matrix synthesis and promotion of changes to cell shape. The gene product has been associated with tumor suppression but has also been correlated with metastasis based on changes to cell shape which can promote tumor cell invasion. [provided by RefSeq, Dec 2011]

Uniprot Description

SPARC: Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites; an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity. Belongs to the SPARC family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q31.3-q32

Cellular Component: extracellular space; platelet alpha granule membrane; nuclear matrix; cell surface; mitochondrion; cytoplasm; plasma membrane; extracellular region; basement membrane

Molecular Function: collagen binding; protein binding; extracellular matrix binding; calcium ion binding

Biological Process: response to gravity; response to peptide hormone stimulus; platelet activation; receptor-mediated endocytosis; extracellular matrix organization and biogenesis; ossification; response to cAMP; heart development; response to glucocorticoid stimulus; response to lipopolysaccharide; signal transduction; response to L-ascorbic acid; negative regulation of angiogenesis; response to ethanol; response to cadmium ion; platelet degranulation; response to cytokine stimulus; negative regulation of endothelial cell proliferation; response to lead ion; regulation of cell morphogenesis; blood coagulation; response to calcium ion; inner ear development; lung development

Disease: Osteogenesis Imperfecta, Type Xvii