Typical Testing Data/Standard Curve (for reference only)
Canine Oxytocin-neurophysin 1 ELISA Kit | ON-1 elisa kit
Canine Oxytocin-neurophysin 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: OTX1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-OTX1 antibody and an OTX1-HRP conjugate. The assay sample and buffer are incubated together with OTX1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OTX1 concentration since OTX1 from samples and OTX1-HRP conjugate compete for the anti-OTX1 antibody binding site. Since the number of sites is limited, as more sites are occupied by OTX1 from the sample, fewer sites are left to bind OTX1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OTX1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a precursor protein that is processed to produce oxytocin and neurophysin I. Oxytocin is a posterior pituitary hormone which is synthesized as an inactive precursor in the hypothalamus along with its carrier protein neurophysin I. Together with neurophysin, it is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. The precursor seems to be activated while it is being transported along the axon to the posterior pituitary. This hormone contracts smooth muscle during parturition and lactation. It is also involved in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. [provided by RefSeq, Dec 2013]
Uniprot Description
OXT: Neurophysin 1 specifically binds oxytocin. Belongs to the vasopressin/oxytocin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 20p13
Cellular Component: extracellular space; extracellular region; terminal button; secretory granule
Molecular Function: oxytocin receptor binding; neurohypophyseal hormone activity
Biological Process: response to peptide hormone stimulus; male mating behavior; response to cAMP; heart development; drinking behavior; response to glucocorticoid stimulus; positive regulation of female receptivity; female pregnancy; signal transduction; positive regulation of synaptic transmission; response to estradiol stimulus; elevation of cytosolic calcium ion concentration; hyperosmotic salinity response; negative regulation of blood pressure; response to electrical stimulus; response to food; grooming behavior; response to retinoic acid; regulation of heart rate; eating behavior; maternal behavior; response to amphetamine; social behavior; response to ether; sleep; response to cocaine; positive regulation of ossification; memory; response to sucrose stimulus; positive regulation of synaptogenesis; positive regulation of prostaglandin secretion; response to activity; regulation of sensory perception of pain; sperm ejaculation; response to progesterone stimulus; positive regulation of blood pressure
Research Articles on ON-1
Similar Products
Product Notes
The Canine ON-1 oxt (Catalog #AAA7202181) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7202181 ELISA Kit recognizes Canine ON-1. It is sometimes possible for the material contained within the vial of "Oxytocin-neurophysin 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
