Related Product Information for OLFM4 elisa kit
Principle of the assay: OLFM-4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for OLFM-4. Standards or samples are then added to the microtiter plate wells and OLFM-4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of OLFM-4 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for OLFM-4 are added to each well to "sandwich" the OLFM-4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain OLFM-4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OLFM-4 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for OLFM4 elisa kit
NCBI Description
This gene was originally cloned from human myeloblasts and found to be selectively expressed in inflammed colonic epithelium. This gene encodes a member of the olfactomedin family. The encoded protein is an antiapoptotic factor that promotes tumor growth and is an extracellular matrix glycoprotein that facilitates cell adhesion. [provided by RefSeq, Mar 2011]
Uniprot Description
OLFM4: May promote proliferation of pancreatic cancer cells by favoring the transition from the S to G2/M phase. In myeloid leukemic cell lines, inhibits cell growth and induces cell differentiation and apoptosis. May play a role in the inhibition of EIF4EBP1 phosphorylation/deactivation. Facilitates cell adhesion, most probably through interaction with cell surface lectins and cadherin.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 13q14.3
Cellular Component: extracellular space; specific granule; azurophil granule; mitochondrion; perinuclear region of cytoplasm; plasma membrane; secretory granule
Molecular Function: protein homodimerization activity; cadherin binding; catalytic activity
Biological Process: regulation of apoptosis; metabolic process; negative regulation of immune response; negative regulation of I-kappaB kinase/NF-kappaB cascade; cell adhesion; regulation of phagocytosis; protein homooligomerization
Product Notes
The Human OLFM4 olfm4 (Catalog #
AAA720253) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA720253 ELISA Kit recognizes Human OLFM4.
It is sometimes possible for the material contained within the vial of
"Olfactomedin 4, ELISA Kit" to become dispersed throughout the inside of
the vial, particularly around the seal of said vial, during shipment and storage. We always
suggest centrifuging these vials
to consolidate all of the liquid away from the lid and to the bottom of the vial prior to
opening. Please be advised that
certain products may require dry ice for shipping and that, if this is the case, an
additional dry ice fee may also be
required.
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