NUDT2 recombinant protein

NUDT2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121186

• Gene Names: NUDT2; APAH1
• Purity: >97% as determined by SDS-PAGE
• Synonyms: NUDT2; NUDT2 Protein; Human; Recombinant (His Tag); Human NUDT2/Ap4A hydrolase Protein (His Tag); APAH1 Protein; nudix (nucleoside diphosphate linked moiety X)-type motif 2; NUDT2 recombinant protein

• Host: E Coli
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala147

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human NUDT2 (P50583)(Met 1-Ala 147) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

References

McLennan AG, et al.,1998, Genomics 47(2): 307-9. Aldenhoven,J. et al.,2003, Cytogenet Genome Res.102 (1-4):121-7. Oka,K. et al., 2011, Int J Cancer. 128 (8):1770-82.

NCBI and Uniprot Product Information

• NCBI GI #: 4502125
• NCBI GeneID: 318
• NCBI Accession #: NP_001152.1
• NCBI GenBank Nucleotide #: NM_001161.4
• UniProt Accession #: P50583; Q5T589; D3DRM0
• Molecular Weight: 16,829 Da
• NCBI Official Full Name: bis(5'-nucleosyl)-tetraphosphatase
• NCBI Official Synonym Full Names: nudix (nucleoside diphosphate linked moiety X)-type motif 2
• NCBI Official Symbol: NUDT2
• NCBI Official Synonym Symbols: APAH1
• NCBI Protein Information: bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]; bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]; Ap4A hydrolase 1; Ap4Aase; bis(5'-nucleosyl)-tetraphosphatase (asymmetrical); diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase; diadenosin
• UniProt Protein Name: Bis(5'-nucleosyl)-tetraphosphatase [asymmetrical]
• Protein Family: Nudix hydrolase
• UniProt Gene Name: NUDT2
• UniProt Synonym Gene Names: APAH1; Ap4A hydrolase; Ap4Aase; Diadenosine tetraphosphatase; Nudix motif 2
• UniProt Entry Name: AP4A_HUMAN

NCBI Description

This gene encodes a member of the MutT family of nucleotide pyrophosphatases, a subset of the larger NUDIX hydrolase family. The gene product possesses a modification of the MutT sequence motif found in certain nucleotide pyrophosphatases. The enzyme asymmetrically hydrolyzes Ap4A to yield AMP and ATP and is responsible for maintaining the intracellular level of the dinucleotide Ap4A, the function of which has yet to be established. This gene may be a candidate tumor suppressor gene. Alternative splicing has been observed at this locus and four transcript variants, all encoding the same protein, have been identified. [provided by RefSeq, Sep 2011]

Uniprot Description

NUDT2: Asymmetrically hydrolyzes Ap4A to yield AMP and ATP. Plays a major role in maintaining homeostasis. Belongs to the Nudix hydrolase family.

Protein type: Hydrolase; Nucleotide Metabolism - pyrimidine; Apoptosis; Nucleotide Metabolism - purine; EC 3.6.1.17

Chromosomal Location of Human Ortholog: 9p13

Cellular Component: mitochondrion

Molecular Function: GTP binding; bis(5'-nucleosyl)-tetraphosphatase (asymmetrical) activity; bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity

Biological Process: apoptosis; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process