Mouse High affinity nerve growth factor receptor ELISA Kit | Ntrk1 elisa kit

Mouse High affinity nerve growth factor receptor ELISA Kit

Cat. Num. AAA761054

• Gene Names: NTRK1; MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
• Reactivity: Mouse
• Synonyms: High affinity nerve growth factor receptor; Mouse High affinity nerve growth factor receptor ELISA Kit; Ntrk1/NTRK-1/MTChigh affinity nerve growth factor receptor/Neurotrophic tyrosine kinase receptor type 1/neurotrophic tyrosine kinase; receptor; type 1/p140-TrkA/TRK1-transforming tyrosine kinase protein/Trk-A/TRKAOncogene TRK/TRKTRK1/tyrosine kinase receptor A; Ntrk1 elisa kit

• Reactivity: Mouse
• Sequence Length: 790

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Ntrk1 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Ntrk1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Ntrk1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Ntrk1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Ntrk1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 59889558
• NCBI GeneID: 4914
• NCBI Accession #: NP_001012331.1
• NCBI GenBank Nucleotide #: NM_001012331.1
• Molecular Weight: 77,145 Da
• NCBI Official Full Name: high affinity nerve growth factor receptor isoform 1
• NCBI Official Synonym Full Names: neurotrophic tyrosine kinase, receptor, type 1
• NCBI Official Symbol: NTRK1
• NCBI Official Synonym Symbols: MTC; TRK; TRK1; TRKA; Trk-A; p140-TrkA
• NCBI Protein Information: high affinity nerve growth factor receptor
• UniProt Protein Name: High affinity nerve growth factor receptor
• Protein Family: High affinity nerve growth factor receptor
• UniProt Gene Name: NTRK1
• UniProt Synonym Gene Names: MTC; TRK; TRKA; Trk-A
• UniProt Entry Name: NTRK1_HUMAN

NCBI Description

This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008]

Uniprot Description

TrkA: a receptor tyrosine kinase of the Trk family. High affinity receptor for nerve growth factor, neurotrophin-3 and neurotrophin-4/5 but not brain- derived neurotrophic factor. Known substrates include Shc, PI-3K, and PLC-gamma-1. Has a crucial role in the development and function of the nociceptive reception system. Two splice variant isoforms have been described.

Protein type: Protein kinase, TK; Membrane protein, integral; Oncoprotein; Protein kinase, tyrosine (receptor); Kinase, protein; EC 2.7.10.1; TK group; Trk family

Chromosomal Location of Human Ortholog: 1q21-q22

Cellular Component: axon; cell soma; cell surface; cytoplasmic vesicle; dendrite; early endosome; early endosome membrane; endosome; integral to plasma membrane; late endosome; late endosome membrane; plasma membrane; protein complex; receptor complex

Molecular Function: ATP binding; GPI-linked ephrin receptor activity; kinase binding; nerve growth factor binding; nerve growth factor receptor activity; neurotrophin binding; neurotrophin p75 receptor binding; protein binding; protein homodimerization activity; transmembrane receptor protein tyrosine kinase activity

Biological Process: activation of MAPKK activity; activation of NF-kappaB transcription factor; adenylate cyclase activation; aging; axon guidance; B cell differentiation; circadian rhythm; detection of mechanical stimulus involved in sensory perception of pain; detection of temperature stimulus involved in sensory perception of pain; ephrin receptor signaling pathway; learning and/or memory; mechanoreceptor differentiation; negative regulation of cell proliferation; negative regulation of neuron apoptosis; nerve growth factor receptor signaling pathway; olfactory nerve development; peptidyl-tyrosine phosphorylation; phosphoinositide-mediated signaling; phospholipase C activation; positive regulation of angiogenesis; positive regulation of GTPase activity; positive regulation of programmed cell death; positive regulation of protein amino acid phosphorylation; positive regulation of Ras protein signal transduction; positive regulation of synaptic transmission, glutamatergic; positive regulation of synaptogenesis; protein amino acid autophosphorylation; protein amino acid phosphorylation; Ras protein signal transduction; response to activity; response to axon injury; response to drug; response to electrical stimulus; response to ethanol; response to hydrostatic pressure; response to nutrient levels; response to radiation; Sertoli cell development; small GTPase mediated signal transduction; sympathetic nervous system development; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Insensitivity To Pain, Congenital, With Anhidrosis; Thyroid Carcinoma, Familial Medullary