5'-Nucleotidase, Cytosolic III (NT5C3) Recombinant Protein | NT5C3 recombinant protein
Recombinant 5'-Nucleotidase, Cytosolic III (NT5C3)
MGHHHHHHSGSEF-MTNQESAVHL KMMPEFQKSS VRIKNPTRVE EIICGLIKGG AAKLQIITDF DMTLSRFSYN GKRCPTCHNI IDNCKLVTDE CRRKLLQLKE QYYAIEVDPV LTVEEKFPYM VEWYTKSHGL LIEQGIPKAK LKEIVADSDV MLKEGYENFF GKLQQHGIPV FIFSAGIGDV LEEVIRQAGV YHSNVKVVSN FMDFDENGVL KGFKGELIHV FNKHDGALKN TDYFSQLKDN SNIILLGDSQ GDLRMADGVA NVEHILKIGY LNDRVDELLE KYMDSYDIVL VKEESLEVVN SILQKTL
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the 5'-nucleotidase family of enzymes that catalyze the dephosphorylation of nucleoside 5'-monophosphates. The encoded protein is the type 1 isozyme of pyrimidine 5' nucleotidase and catalyzes the dephosphorylation of pyrimidine 5' monophosphates. Mutations in this gene are a cause of hemolytic anemia due to uridine 5-prime monophosphate hydrolase deficiency. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and pseudogenes of this gene are located on the long arm of chromosomes 3 and 4. [provided by RefSeq, Mar 2012]
Uniprot Description
NT5C3A: Can act both as nucleotidase and as phosphotransferase. Defects in NT5C3 are the cause of P5N deficiency (P5ND); also called hemolytic anemia due to P5N deficiency or hemolytic anemia due to UMPH1 deficiency. P5ND is an autosomal recessive condition causing hemolytic anemia characterized by marked basophilic stipplig and the accumulation of high concentrations of pyrimidine nucleotides within the erythrocyte. It is implicated in the anemia of lead poisoning and is possibly associated with learning difficulties. Belongs to the pyrimidine 5'-nucleotidase family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Phosphatase (non-protein); EC 3.1.3.5; Nucleotide Metabolism - purine; Nucleotide Metabolism - pyrimidine; Transferase; Endoplasmic reticulum
Chromosomal Location of Human Ortholog: 7p14.3
Cellular Component: mitochondrion; endoplasmic reticulum; cytoplasm; cytosol
Molecular Function: 5'-nucleotidase activity; 2'-phosphotransferase activity; nucleotide binding; magnesium ion binding
Biological Process: pyrimidine nucleoside catabolic process; pyrimidine base metabolic process; dephosphorylation; nucleobase, nucleoside and nucleotide metabolic process; nucleotide metabolic process; adenosine metabolic process; pyrimidine nucleoside metabolic process
Disease: Uridine 5-prime Monophosphate Hydrolase Deficiency, Hemolytic Anemia Due To
Research Articles on NT5C3
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Product Notes
The NT5C3 nt5c3a (Catalog #AAA2011361) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's 5'-Nucleotidase, Cytosolic III (NT5C3) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the NT5C3 nt5c3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF-MTNQES AVHL KMMPEFQKSS VRIKNPTRVE EIICGLIKGG AAKLQIITDF DMTLSRFSYN GKRCPTCHNI IDNCKLVTDE CRRKLLQLKE QYYAIEVDPV LTVEEKFPYM VEWYTKSHGL LIEQGIPKAK LKEIVADSDV MLKEGYENFF GKLQQHGIPV FIFSAGIGDV LEEVIRQAGV YHSNVKVVSN FMDFDENGVL KGFKGELIHV FNKHDGALKN TDYFSQLKDN SNIILLGDSQ GDLRMADGVA NVEHILKIGY LNDRVDELLE KYMDSYDIVL VKEESLEVVN SILQKTL. It is sometimes possible for the material contained within the vial of "5'-Nucleotidase, Cytosolic III (NT5C3), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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