Horse N-Terminal Pro Atrial Natriuretic Peptide ELISA Kit | NT-PROANP elisa kit

Horse N-Terminal Pro Atrial Natriuretic Peptide ELISA Kit

Cat. Num. AAA45977

• Gene Names: NPPA; ANF; ANP; PND; ATFB6; ATRST2; CDD-ANF
• Reactivity: Horse
• Synonyms: N-Terminal Pro Atrial Natriuretic Peptide; Horse N-Terminal Pro Atrial Natriuretic Peptide ELISA Kit; NT-PROANP elisa kit

• Reactivity: Horse
• Sequence Length: 151

• Samples: Serum, Plasma, Cell Culture Supernatants, Tissue Homogenate, Urine and other Body Fluids
• Detection Range: 50 pg/ml ~ 1600 pg/ml.
• Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Horse NT-PROANP in the specimen, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the specimens and allow the operator to produce a standard curve of Optical Density versus Horse NT-PROANP concentration. The concentration of Horse NT-PROANP in the specimens is then determined by comparing the O.D. of the specimens to the standard curve.
• Intended Uses: This ELISA kit is intended for laboratory in vitro research use only, not for drug, household or other use! The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm by a spectrophotometer. In order to measure the concentration of Horse NT-PROANP in the specimen, this ELISA kit includes a set of calibration standards. The calibration standards are assayed at the same time as the specimens and allow the operator to produce a standard curve of Optical Density versus Horse NT-PROANP concentration. The concentration of Horse NT-PROANP in the specimens is then determined by comparing the O.D. of the specimens to the standard curve.
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NT-PROANP elisa kit

Background: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of specimens. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the specimen.

NCBI and Uniprot Product Information

• NCBI GI #: 23510319
• NCBI GeneID: 4878
• NCBI Accession #: NP_006163.1
• NCBI GenBank Nucleotide #: NM_006172.3
• UniProt Accession #: P01160; Q13766; Q5JZE1
• Molecular Weight: 16,708 Da
• NCBI Official Full Name: natriuretic peptides A preproprotein
• NCBI Official Synonym Full Names: natriuretic peptide A
• NCBI Official Symbol: NPPA
• NCBI Official Synonym Symbols: ANF; ANP; PND; ATFB6; ATRST2; CDD-ANF
• NCBI Protein Information: natriuretic peptides A; atriopeptin; cardionatrin; prepronatriodilatin; natriuretic peptide precursor A
• UniProt Protein Name: Natriuretic peptides A
• UniProt Gene Name: NPPA
• UniProt Synonym Gene Names: ANP; PND; CDP; ANF; ANP
• UniProt Entry Name: ANF_HUMAN

NCBI Description

The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq, Sep 2009]

Uniprot Description

NPPA: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Defects in NPPA are the cause of familial atrial fibrillation type 6 (ATFB6). Atrial fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of patients. It is characterized by disorganized atrial electrical activity, progressive deterioration of atrial electromechanical function and ineffective pumping of blood into the ventricles. It can be associated with palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the natriuretic peptide family.

Protein type: Hormone; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1p36.21

Cellular Component: extracellular space; mast cell granule; perinuclear region of cytoplasm; extracellular region; nucleus

Molecular Function: neuropeptide hormone activity; peptide hormone receptor binding; receptor binding

Biological Process: cardiac muscle hypertrophy; transcription initiation from RNA polymerase II promoter; cGMP biosynthetic process; negative regulation of systemic arterial blood pressure; positive regulation of heart rate; female pregnancy; response to insulin stimulus; neuropeptide signaling pathway; regulation of blood pressure; receptor guanylyl cyclase signaling pathway; response to hypoxia; gene expression; negative regulation of cell growth; regulation of blood vessel size

Disease: Atrial Standstill 2; Atrial Fibrillation, Familial, 6