Structure
NSC 95397, Inhibitor | NSC95397, inhibitor
NSC 95397
Purity
97.12%
Synonyms
NSC 95397; NSC95397; inhibitor
Purity/Purification
97.12%
Form/Format
Solid. Powder
Solubility
DMSO: 100mg/mL (322.18mM)
(<1mg/ml refers to the product slightly soluble or insoluble)
(<1mg/ml refers to the product slightly soluble or insoluble)
Formula
C14H14O4S2
SMILES
O=C1C(SCCO)=C(SCCO)C(=O)c2ccccc21
CAS Number
93718-83-3
Target & IC50
Cdc25A: ic50 32nM (ki)
Cdc25B: ic50 96nM (ki)
Cdc25C: ic50 40nM (ki)
Cdc25B: ic50 96nM (ki)
Cdc25C: ic50 40nM (ki)
Cell Research
To measure the cell proliferation activity of NSC 95397 against colon cancer cells, SW480 (1 x 10^4), SW620 (2 x 10^4), and DLD-1 (1 x 10^4) cells were seeded into 96-well plates. After overnight culture, NSC 95397 cells were added at the indicated concentrations. After 24 hours of incubation, cell proliferation was determined in vitro using a BrdU cell proliferation assay kit. O.D. 450 values were analyzed by using a Multiskan PC. The apoptosis of colon cancer cells was determined by a PE Annexin V Apoptosis Detection Kit with 7-AAD. Briefly, cells were treated with the indicated concentration of NSC 95397 for 24 hours. After 24 h, Cells were harvested and stained with PE Annexin V/7-AAD for 15 minutes. The stained cells were analyzed using FACSCanto II low cytometer and FCS Express software[1]
In Vitro
NSC 95397 reduced cell viability and anchorage-independent growth of all the three colon cancer cell lines through inhibited proliferation and induced apoptosis via regulating cell-cycle-related proteins, including p21, cyclin-dependent kinases, and caspases[1].
Preparation and Storage
Store at -20 degree C for 3 years powder
-80 degree C for 2 years in solvent
-80 degree C for 2 years in solvent
Structure
Structure
Related Product Information for NSC95397, inhibitor
NSC 95397 is a potent inhibitor of Cdc25 dual specificity phosphatase(Ki of 32, 96, and 40nM for Cdc25A, Cdc25B, and Cdc25C, respectively).
Product Categories/Family for NSC95397, inhibitor
References
Navneet D, Bou-Yue P, Chien-Min L, et al. NSC 95397 Suppresses Proliferation and Induces Apoptosis in Colon Cancer Cells through MKP-1 and the ERK1/2 Pathway[J]. International Journal of Molecular Sciences, 2018, 19(6):1625-.
Navneet D, Bou-Yue P, Chien-Min L, et al. NSC 95397 Suppresses Proliferation and Induces Apoptosis in Colon Cancer Cells through MKP-1 and the ERK1/2 Pathway[J]. International Journal of Molecular Sciences, 2018, 19(6):1625-.
Park H, Carr B I, Li M, et al. Fluorinated NSC as a Cdc25 inhibitor[J]. Bioorganic and Medicinal Chemistry Letters, 2007, 17(8):2351-2354.
Park H, Carr B I, Li M, et al. Fluorinated NSC as a Cdc25 inhibitor[J]. Bioorganic and Medicinal Chemistry Letters, 2007, 17(8):2351-2354.
Dubey NK, et al. NSC 95397 Suppresses Proliferation and Induces Apoptosis in Colon Cancer Cells through MKP-1 and the ERK1/2 Pathway. Int J Mol Sci. 2018 May 31;19(6). pii: E1625.
Navneet D, Bou-Yue P, Chien-Min L, et al. NSC 95397 Suppresses Proliferation and Induces Apoptosis in Colon Cancer Cells through MKP-1 and the ERK1/2 Pathway[J]. International Journal of Molecular Sciences, 2018, 19(6):1625-.
Park H, Carr B I, Li M, et al. Fluorinated NSC as a Cdc25 inhibitor[J]. Bioorganic and Medicinal Chemistry Letters, 2007, 17(8):2351-2354.
Park H, Carr B I, Li M, et al. Fluorinated NSC as a Cdc25 inhibitor[J]. Bioorganic and Medicinal Chemistry Letters, 2007, 17(8):2351-2354.
Dubey NK, et al. NSC 95397 Suppresses Proliferation and Induces Apoptosis in Colon Cancer Cells through MKP-1 and the ERK1/2 Pathway. Int J Mol Sci. 2018 May 31;19(6). pii: E1625.
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Product Notes
The NSC95397 (Catalog #AAA5758285) is an Inhibitor and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "NSC 95397, Inhibitor" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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