Nuclear receptor subfamily 2 group C member 2 (Nr2c2) Recombinant Protein | Nr2c2 recombinant protein

Recombinant Human Nuclear receptor subfamily 2 group C member 2 (Nr2c2), partial

Cat. Num. AAA1265235

• Gene Names: NR2C2; TR4; TAK1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Nuclear receptor subfamily 2 group C member 2 (Nr2c2); Recombinant Human Nuclear receptor subfamily 2 group C member 2 (Nr2c2); partial; Orphan nuclear receptor TAK1Orphan nuclear receptor TR4Testicular receptor 4; Nr2c2 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 3-592aa, Partial
• Sequence: SPSPRIQIISTDSAVASPQRIQIVTDQQTGQKIQIVTAVDASGSPKQQFILTSPDGAGTGKVILASPETSSAKQLIFTTSDNLVPGRIQIVTDSASVERLLGKTDVQRPQVVEYCVVCGDKASGRHYGAVSCEGCKGFFKRSVRKNLTYSCRSNQDCIINKHHRNRCQFCRLKKCLEMGMKMESVQSERKPFDVQREKPSNCAASTEKIYIRKDLRSPLIATPTFVADKDGARQTGLLDPGMLVNIQQPLIREDGTVLLATDSKAETSQGALGTLANVVTSLANLSESLNNGDTSEIQPEDQSASEITRAFDTLAKALNTTDSSSSPSLADGIDTSGGGSIHVISRDQSTPIIEVEGPLLSDTHVTFKLTMPSPMPEYLNVHYICESASRLLFLSMHWARSIPAFQALGQDCNTSLVRACWNELFTLGLAQCAQVMSLSTILAAIVNHLQNSIQEDKLSGDRIKQVMEHIWKLQEFCNSMAKLDIDGYEYAYLKAIVLFSPDHPGLTSTSQIEKFQEKAQMELQDYVQKTYSEDTYRLARILVRLPALRLMSSNITEELFFTGLIGNVSIDSIIPYILKMETAEYNGQIT

• Species: Homo sapiens (Human)
• Subcellular Location: Nucleus
• Protein Families: Nuclear hormone receptor family, NR2 subfamily
• Tissue Specificity: Expressed, during embryogenesis, in perichondrium, developing glomeruli structures and tubules of kidney, as well as in intestiinal villi. Also expressed in lung and hair follicles.
• Relevance: Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear recptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during expression during the late phase of spermatogenesis. Activates transcriptional activity of LHCG and is antagonist of PPARA-mediated transactivation. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses bryonic and fetal globin transcription including that of GATA1. Binds to hormone response elents (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early bryonic development and bryonic st cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior
• Function: Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear receptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Activates transcriptional activity of LHCG and is antagonist of PPARA-mediated transactivation (By similarity). Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Preparation and Storage: Store at -20 degree C. For extended storage, store at -20 or -80 degree C.

Product Categories/Family for Nr2c2 recombinant protein

Developmental Biology

References

Cloning of the gene encoding the murine orphan receptor TAK1 and cell-type-specific expression in testis.Hirose T., O'Brien D.A., Jetten A.M.Gene 163:239-242(1995)
https://www.ncbi.nlm.nih.gov/UniGene/clust.cgi?ORG=Mm&CID=442385
https://www.genome.jp/dbget-bin/www_bget?mmu:22026
https://string-db.org/network/10090.ENSMUSP00000109087

NCBI and Uniprot Product Information

• NCBI GI #: 36950991
• NCBI GeneID: 7182
• NCBI Accession #: NP_003289.2
• NCBI GenBank Nucleotide #: NM_003298.4
• UniProt Accession #: P49116; P55092; A8K3H5; B6ZGT8
• Molecular Weight: 67,345 Da
• NCBI Official Full Name: nuclear receptor subfamily 2 group C member 2 isoform 1
• NCBI Official Synonym Full Names: nuclear receptor subfamily 2, group C, member 2
• NCBI Official Symbol: NR2C2
• NCBI Official Synonym Symbols: TR4; TAK1
• NCBI Protein Information: nuclear receptor subfamily 2 group C member 2; orphan nuclear receptor TR4; Nuclear hormone receptor TR4; orphan nuclear receptor TAK1; testicular nuclear receptor 4
• UniProt Protein Name: Nuclear receptor subfamily 2 group C member 2
• Protein Family: Nuclear receptor subfamily
• UniProt Gene Name: NR2C2
• UniProt Synonym Gene Names: TAK1; TR4
• UniProt Entry Name: NR2C2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the nuclear hormone receptor family. Members of this family act as ligand-activated transcription factors and function in many biological processes such as development, cellular differentiation and homeostasis. The activated receptor/ligand complex is translocated to the nucleus where it binds to hormone response elements of target genes. The protein encoded by this gene plays a role in protecting cells from oxidative stress and damage induced by ionizing radiation. The lack of a similar gene in mouse results in growth retardation, severe spinal curvature, subfertility, premature aging, and prostatic intraepithelial neoplasia (PIN) development. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2014]

Uniprot Description

NR2C2: Orphan nuclear receptor that can act as a repressor or activator of transcription. An important repressor of nuclear recptor signaling pathways such as retinoic acid receptor, retinoid X, vitamin D3 receptor, thyroid hormone receptor and estrogen receptor pathways. May regulate gene expression during the late phase of spermatogenesis. Together with NR2C1, forms the core of the DRED (direct repeat erythroid-definitive) complex that represses embryonic and fetal globin transcription including that of GATA1. Binds to hormone response elements (HREs) consisting of two 5'-AGGTCA-3' half site direct repeat consensus sequences. Plays a fundamental role in early embryonic development and embryonic stem cells. Required for normal spermatogenesis and cerebellum development. Appears to be important for neurodevelopmentally regulated behavior. Activates transcriptional activity of LHCG. Antagonist of PPARA-mediated transactivation. Belongs to the nuclear hormone receptor family. NR2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor; Nuclear receptor

Chromosomal Location of Human Ortholog: 3p25

Cellular Component: nucleoplasm

Molecular Function: protein binding; zinc ion binding; protein heterodimerization activity; sequence-specific DNA binding; transcription coactivator activity; steroid hormone receptor activity; receptor activity; transcription factor activity

Biological Process: nervous system development; transcription initiation from RNA polymerase II promoter; positive regulation of embryonic development; regulation of transcription, DNA-dependent; meiotic cell cycle; positive regulation of behavior; cerebellum development; steroid hormone mediated signaling; gene expression; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; cell differentiation