Human N-Methyl-D-Aspartate ELISA Kit | NR2 elisa kit
Human N-Methyl-D-Aspartate (NMDA) Receptor 2 (NR2) ELISA Kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate NR2 in Human serum, plasma. An antibody specific for NR2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NR2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NR2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NR2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
N-methyl-D-aspartate (NMDA) receptors are a class of ionotropic glutamate receptors. NMDA receptor channel has been shown to be involved in long-term potentiation, an activity-dependent increase in the efficiency of synaptic transmission thought to underlie certain kinds of memory and learning. NMDA receptor channels are heteromers composed of three different subunits: NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The NR2 subunit acts as the agonist binding site for glutamate. This receptor is the predominant excitatory neurotransmitter receptor in the mammalian brain. [provided by RefSeq, Jul 2008]
Uniprot Description
NMDAR2B: an NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. Plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. Mediates neuronal functions in glutamate neurotransmission. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death.
Protein type: Channel, ligand-gated; Channel, calcium; Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 12p12
Cellular Component: cell junction; cell surface; integral to plasma membrane; N-methyl-D-aspartate selective glutamate receptor complex; neuron projection; plasma membrane; postsynaptic membrane; synaptic vesicle
Molecular Function: calcium channel activity; extracellular-glutamate-gated ion channel activity; glycine binding; N-methyl-D-aspartate selective glutamate receptor activity; protein binding; zinc ion binding
Biological Process: activation of MAPKK activity; axon guidance; behavioral fear response; behavioral response to pain; detection of mechanical stimulus involved in sensory perception of pain; ephrin receptor signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; glutamate signaling pathway; in utero embryonic development; innate immune response; insulin receptor signaling pathway; ionotropic glutamate receptor signaling pathway; learning; learning and/or memory; MAPKKK cascade; memory; nerve growth factor receptor signaling pathway; Ras protein signal transduction; regulation of excitatory postsynaptic membrane potential; response to ethanol; sensory organ development; small GTPase mediated signal transduction; startle response; suckling behavior; synaptic transmission; transport; vascular endothelial growth factor receptor signaling pathway
Disease: Epileptic Encephalopathy, Early Infantile, 27; Mental Retardation, Autosomal Dominant 6
Research Articles on NR2
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Product Notes
The Human NR2 grin2b (Catalog #AAA284106) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA284106 ELISA Kit recognizes Human NR2. It is sometimes possible for the material contained within the vial of "N-Methyl-D-Aspartate, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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