LXRb recombinant protein

Recombinant Human LXRb protein

Cat. Num. AAA1561062

• Gene Names: NR1H2; NER; UNR; LXRB; LXR-b; NER-I; RIP15
• Purity: >90% as determined by SDS-PAGE
• Synonyms: LXRb; Recombinant Human LXRb protein; Oxysterols receptor LXR-beta; Liver X receptor beta; Nuclear receptor NER; Nuclear receptor subfamily 1 group H member 2; Ubiquitously-expressed nuclear receptor; LXRb recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human LXRb (Lys72-Glu460) was fused with His tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for LXRb recombinant protein

Nuclear receptor that exhibits a ligand-dependent transcriptional activation activity. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8; DLDLR and LRP8. Interplays functionally with RORA for the regulation of genes involved in liver metabolism. Induces LPCAT3-dependent phospholipid remodeling in endoplasmic reticulum (ER) membranes of hepatocytes, driving SREBF1 processing and lipogenesis (By similarity). Via LPCAT3, triggers the incorporation of arachidonate into phosphatidylcholines of ER membranes, increasing membrane dynamics and enabling triacylglycerols transfer to nascent very low-density lipoprotein (VLDL) particles. Via LPCAT3 also counteracts lipid-induced ER stress response and inflammation, likely by modulating SRC kinase membrane compartmentalization and limiting the synthesis of lipid inflammatory mediators. Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex.

Product Categories/Family for LXRb recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 375331888
• NCBI GeneID: 7376
• NCBI Accession #: NP_001243576.1
• NCBI GenBank Nucleotide #: NM_001256647.1
• UniProt Accession #: P55055; Q12970; Q5I0Y1; A8K490; B4DNM6; E7EWA6
• Molecular Weight: 39,920 Da
• NCBI Official Full Name: oxysterols receptor LXR-beta isoform 2
• NCBI Official Synonym Full Names: nuclear receptor subfamily 1, group H, member 2
• NCBI Official Symbol: NR1H2
• NCBI Official Synonym Symbols: NER; UNR; LXRB; LXR-b; NER-I; RIP15
• NCBI Protein Information: oxysterols receptor LXR-beta; LX receptor beta; liver X nuclear receptor beta; nuclear orphan receptor LXR-beta; nuclear receptor NER; steroid hormone-nuclear receptor NER; ubiquitously-expressed nuclear receptor
• UniProt Protein Name: Oxysterols receptor LXR-beta
• UniProt Gene Name: NR1H2
• UniProt Synonym Gene Names: LXRB; NER; UNR
• UniProt Entry Name: NR1H2_HUMAN

NCBI Description

The liver X receptors, LXRA (NR1H3; MIM 602423) and LXRB, form a subfamily of the nuclear receptor superfamily and are key regulators of macrophage function, controlling transcriptional programs involved in lipid homeostasis and inflammation. The inducible LXRA is highly expressed in liver, adrenal gland, intestine, adipose tissue, macrophages, lung, and kidney, whereas LXRB is ubiquitously expressed. Ligand-activated LXRs form obligate heterodimers with retinoid X receptors (RXRs; see MIM 180245) and regulate expression of target genes containing LXR response elements (summary by Korf et al., 2009 [PubMed 19436111]).[supplied by OMIM, Jan 2010]

Uniprot Description

LXR-beta: Orphan receptor. Binds preferentially to double-stranded oligonucleotide direct repeats having the consensus half-site sequence 5'-AGGTCA-3' and 4-nt spacing (DR-4). Regulates cholesterol uptake through MYLIP-dependent ubiquitination of LDLR, VLDLR and LRP8. Forms a heterodimer with RXR. Ubiquitous. Belongs to the nuclear hormone receptor family. NR1 subfamily.

Protein type: Nuclear receptor

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: retinoid X receptor binding; ligand-dependent nuclear receptor activity; protein binding; DNA binding; zinc ion binding; steroid hormone receptor activity; apolipoprotein A-I receptor binding; ATPase binding

Biological Process: negative regulation of lipid transport; negative regulation of proteolysis; retinoic acid receptor signaling pathway; positive regulation of fatty acid biosynthetic process; transcription initiation from RNA polymerase II promoter; positive regulation of cellular protein metabolic process; cellular lipid metabolic process; negative regulation of pinocytosis; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cholesterol transport; cholesterol homeostasis; positive regulation of lipoprotein lipase activity; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent