Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 ELISA Kit | NOX1 elisa kit

Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 ELISA Kit

Cat. Num. AAA755168

• Gene Names: NOX1; MOX1; NOH1; NOH-1; GP91-2
• Reactivity: Canine
• Synonyms: Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1; Canine Nicotinamide Adenine Dinucleotide Phosphate Oxidase 1 ELISA Kit; NOX1 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This NOX1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine NOX1. This ELISA kit for research use only, not for therapeutic applications
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NOX1 elisa kit

Principle of the Assay: NOX1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-NOX1 antibody and an NOX1-HRP conjugate. The assay sample and buffer are incubated together with NOX1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NOX1 concentration since NOX1 from samples and NOX1-HRP conjugate compete for the anti-NOX1 antibody binding site. Since the number of sites is limited, as more sites are occupied by NOX1 from the sample, fewer sites are left to bind NOX1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NOX1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 148536873
• NCBI GeneID: 27035
• NCBI Accession #: NP_008983.2
• NCBI GenBank Nucleotide #: NM_007052.4
• Molecular Weight: 58,972 Da
• NCBI Official Full Name: NADPH oxidase 1 isoform 1
• NCBI Official Synonym Full Names: NADPH oxidase 1
• NCBI Official Symbol: NOX1
• NCBI Official Synonym Symbols: MOX1; NOH1; NOH-1; GP91-2
• NCBI Protein Information: NADPH oxidase 1; mitogenic oxidase 1; NADPH oxidase homolog-1; NADH/NADPH mitogenic oxidase subunit P65-MOX; mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating)
• UniProt Protein Name: NADPH oxidase 1
• Protein Family: NADPH oxidase
• UniProt Gene Name: NOX1
• UniProt Synonym Gene Names: MOX1; NOH1; NOX-1; MOX-1
• UniProt Entry Name: NOX1_HUMAN

NCBI Description

This gene encodes a member of the NADPH oxidase family of enzymes responsible for the catalytic one-electron transfer of oxygen to generate superoxide or hydrogen peroxide. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]

Uniprot Description

NOX1: NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.-.-.-; Oxidoreductase; Motility/polarity/chemotaxis; Channel, misc.; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: Xq22

Cellular Component: early endosome; integral to membrane; cell junction; NADPH oxidase complex

Molecular Function: protein binding; superoxide-generating NADPH oxidase activity; metal ion binding; Rac GTPase binding; NADP binding; voltage-gated proton channel activity

Biological Process: respiratory burst; intracellular pH elevation; cell migration; extracellular matrix organization and biogenesis; positive regulation of smooth muscle cell proliferation; superoxide metabolic process; positive regulation of JNK cascade; signal transduction; NADP metabolic process; regulation of systemic arterial blood pressure by renin-angiotensin; proton transport; regulation of blood pressure; positive regulation of cell proliferation; hydrogen peroxide metabolic process; positive regulation of integrin biosynthetic process; angiogenesis; inflammatory response; superoxide release