IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human breast cancer tissue using Rabbit anti NFkB p65 (pSer276) antibody at a dilution of 1/100)
Rabbit NFkB Polyclonal Antibody | anti-NFkB antibody
Rabbit Anti NFkB p65 (pSer276)
Gene Names
RELA; p65; CMCU; NFKB3
Reactivity
Human, RatPredicted: Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Purified IgG was prepared from whole serum by affinity chromatography.
Synonyms
NFkB, Antibody; Rabbit Anti NFkB p65 (pSer276); NFkB p65 (pSer276) antibody; anti-NFkB antibody
Host
Rabbit
Reactivity
Human, Rat
Predicted: Mouse
Predicted: Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified IgG was prepared from whole serum by affinity chromatography.
Form/Format
Liquid; Phosphate Buffered Saline
Sequence Length
548
Applicable Applications for anti-NFkB antibody
WB (Western Blot), IHC (Immunohistochemistry)
Immunogen
Phospho specific-peptide corresponding to residues surrounding serine 276 of human NFkB p65
Preservative Stabilizers
0.02% Sodium Azide 50% Glycerol
Preparation and Storage
Store at -20 degree C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human breast cancer tissue using Rabbit anti NFkB p65 (pSer276) antibody at a dilution of 1/100)
IHC (Immunohistochemisry)
(Immunohistochemistry of paraffin-embedded human breast cancer tissue using Rabbit anti NFkB p65 (pSer276) antibody at a dilution of 1/100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded rat testis using Rabbit anti NFkB p65 (pSer276) antibody at a dilution of 1/100)
IHC (Immunohiostchemistry)
(Immunohistochemistry of paraffin-embedded rat testis using Rabbit anti NFkB p65 (pSer276) antibody at a dilution of 1/100)
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells untreated or treated with TNF-alpha using Rabbit anti NFkB p65 (pSer276) antibody. Cells were treated with TNF-alpha (20ng/ml) for 30 minutes. 3% BSA was used for blocking)
WB (Western Blot)
(Western blot analysis of extracts from HeLa cells untreated or treated with TNF-alpha using Rabbit anti NFkB p65 (pSer276) antibody. Cells were treated with TNF-alpha (20ng/ml) for 30 minutes. 3% BSA was used for blocking)
Related Product Information for anti-NFkB antibody
Rabbit anti NFkB p65 (pSer276) antibody recognizes nuclear factor NF-kappa-B p65 subunit (NFkB3 p65) also known as transcription factor p65 or RELA, when phosphorylated at serine 276. NFkB p65 is part of the NFkB transcription factor complex that also includes RELB, NFkB1 p105, NFkB1 p50, REL and NFkB2 p52. NFkB p65 regulates expression of a wide variety of genes involved in immune and inflammatory responses (May, M.J. & Ghosh, S. 1997). Protein kinase A (PKA) phosphorylates NFkB p65 on serine 276 and modulates transcriptional activity of NFkB by mediating its interaction with CBP/p300 (Zhong, H. et al. 1998). Serine 276 has also been demonstrated to be phosphorylated by mitogen- and stress- activated protein kinase-1 (MSK1) (Vermeulen, L. et al. 2003). The NFkB pathway plays a critical role in orchestrating immune responses after infection and has been implicated in a variety of diseases ranging from autoimmune disorders to cancer.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
transcription factor p65 isoform 2
NCBI Official Synonym Full Names
RELA proto-oncogene, NF-kB subunit
NCBI Official Symbol
RELA
NCBI Official Synonym Symbols
p65; CMCU; NFKB3
NCBI Protein Information
transcription factor p65
UniProt Protein Name
Transcription factor p65
UniProt Gene Name
RELA
UniProt Synonym Gene Names
NFKB3
UniProt Entry Name
TF65_HUMAN
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Product Notes
The NFkB rela (Catalog #AAA50320) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti NFkB p65 (pSer276) reacts with Human, Rat Predicted: Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NFkB can be used in a range of immunoassay formats including, but not limited to, WB (Western Blot), IHC (Immunohistochemistry). Researchers should empirically determine the suitability of the NFkB rela for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFkB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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