Nmnat1 recombinant protein

Nmnat1, 1-285aa, Mouse, His-tag, E Coli

Cat. Num. AAA206656

• Gene Names: Nmnat1; nmnat; D4Cole1e; 2610529L11Rik; 5730441G13Rik
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: Nmnat1; 1-285aa; Mouse; His-tag; E Coli; Nicotinamide/nicotinic acid mononucleotide adenylyltransferase1; 2610529L11Rik; 5730441G13Rik; D4Cole1e; nmnat; Nmnat1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 20% glycerol, 1mM EDTA
• Concentration: 1mg/ml (determined by Absorbance 280nm) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMDSSKKT EVVLLACGSF NPITNMHLRL FELAKDYMHA TGKYSVIKGI ISPVGDAYKK KGLIPAHHRI IMAELATKNS HWVEVDTWES LQKEWVETVK VLRYHQEKLA TGSCSYPQSS PALEKPGRKR KWADQKQDSS PQKPQEPKPT GVPKVKLLCG ITNDISSTKI RRALRRGQSI RYLVPDLVQE YIEKHELYNT ESEGRNAGVT LAPLQRNAAE AKHNHSTL
• Sequence Length: 285

• Applicable Applications for Nmnat1 recombinant protein: SDS-PAGE
• Antigen Species: Mouse
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for Nmnat1 recombinant protein

Nmnat1, also known as nicotinamide/nicotinic acid mononucleotide adenylyltransferase1, is a central enzyme in NAD biosynthesis, catalyzing the condensation of nicotinamide mononucleotide (NMN) of nicotinic acid mononucleotide (NaMN) with the AMP moiety of ATP to form NAD or NaAD. It is widely expressed with high levels in skeletal muscle, heart, liver and kidney. This protein appears to have the ability to protect against axonal degeneration following mechanical or toxic insults. Recombinant mouse Nmnat1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for Nmnat1 recombinant protein

Neurological proteins

References

Emanuelli M., et al. (2001) J Biol Chem. 276 (1):406-12; Zhou T., et al. (2002) J Biol Chem. 277 (15) 13148-54.

NCBI and Uniprot Product Information

• NCBI GI #: 57527870
• NCBI GeneID: 66454
• NCBI Accession #: NP_597679
• NCBI GenBank Nucleotide #: NM_133435.2
• UniProt Accession #: Q9EPA7; Q6B504
• Molecular Weight: 34.7 kDa (308aa) confirmed by MALDI-TOF
• NCBI Official Full Name: nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1
• NCBI Official Synonym Full Names: nicotinamide nucleotide adenylyltransferase 1
• NCBI Official Symbol: Nmnat1
• NCBI Official Synonym Symbols: nmnat; D4Cole1e; 2610529L11Rik; 5730441G13Rik
• NCBI Protein Information: nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1
• UniProt Protein Name: Nicotinamide/nicotinic acid mononucleotide adenylyltransferase 1
• UniProt Gene Name: Nmnat1
• UniProt Synonym Gene Names: D4Cole1e; Nmnat; NMN/NaMN adenylyltransferase 1; NMN adenylyltransferase 1; NaMN adenylyltransferase 1

Uniprot Description

Catalyzes the formation of NAD+ from nicotinamide mononucleotide (NMN) and ATP (PubMed:15381699). Can also use the deamidated form; nicotinic acid mononucleotide (NaMN) as substrate with the same efficiency (). Can use triazofurin monophosphate (TrMP) as substrate (). Also catalyzes the reverse reaction, i.e. the pyrophosphorolytic cleavage of NAD+ (). For the pyrophosphorolytic activity, prefers NAD+ and NaAD as substrates and degrades NADH, nicotinic acid adenine dinucleotide phosphate (NHD) and nicotinamide guanine dinucleotide (NGD) less effectively (). Involved in the synthesis of ATP in the nucleus, together with PARP1, PARG and NUDT5 (). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (). Fails to cleave phosphorylated dinucleotides NADP+, NADPH and NaADP+ (). Protects against axonal degeneration following mechanical or toxic insults (PubMed:15310905, PubMed:16914673). Delays axonal degeneration after axotomy. Results in a >10-fold increase in intact neurites 72 hours after injury (PubMed:16914673).