Human Neuroligin-4, X-linked ELISA Kit | NLGN4X elisa kit

Human Neuroligin-4, X-linked ELISA Kit

Cat. Num. AAA764084

• Gene Names: NLGN4X; HLNX; HNL4X; NLGN4; ASPGX2; AUTSX2
• Reactivity: Human
• Synonyms: Neuroligin-4; X-linked; Human Neuroligin-4; X-linked ELISA Kit; NLGN4X/ASPGX2/AUTSX2/HNLX/NL4/NLGN4/ASPGX2/HLNX/HNLX/KIAA1260AUTSX2/MGC22376/neuroligin 4; X-linked/NLGN/NLGN4neuroligin 4/X-linked; NLGN4X elisa kit

• Reactivity: Human
• Sequence Length: 816

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for NLGN4X elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human NLGN4X antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human NLGN4X antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human NLGN4X amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human NLGN4X can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 531034776
• NCBI GeneID: 57502
• NCBI Accession #: NP_001269075.1
• NCBI GenBank Nucleotide #: NM_001282146.1
• Molecular Weight: 94,047 Da
• NCBI Official Full Name: neuroligin-4, X-linked
• NCBI Official Synonym Full Names: neuroligin 4, X-linked
• NCBI Official Symbol: NLGN4X
• NCBI Official Synonym Symbols: HLNX; HNL4X; NLGN4; ASPGX2; AUTSX2
• NCBI Protein Information: neuroligin-4, X-linked
• UniProt Protein Name: Neuroligin-4, X-linked
• Protein Family: Neuroligin
• UniProt Gene Name: NLGN4X
• UniProt Synonym Gene Names: KIAA1260; NLGN4; Neuroligin X
• UniProt Entry Name: NLGNX_HUMAN

NCBI Description

This gene encodes a member of the type-B carboxylesterase/lipase protein family. The encoded protein belongs to a family of neuronal cell surface proteins. Members of this family may act as splice site-specific ligands for beta-neurexins and may be involved in the formation and remodeling of central nervous system synapses. The encoded protein interacts with discs large homolog 4 (DLG4). Mutations in this gene have been associated with autism and Asperger syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

NLGN4X: Putative neuronal cell surface protein involved in cell- cell-interactions. Defects in NLGN4X may be the cause of susceptibility to autism X-linked type 2 (AUTSX2). AUTSX2 is a pervasive developmental disorder (PDD), prototypically characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age. Defects in NLGN4X may be the cause of susceptibility to X-linked Asperger syndrome 2 (ASPGX2). ASPGX2 is considered to be a form of childhood autism. Belongs to the type-B carboxylesterase/lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.33

Cellular Component: cell junction; cell surface; dendrite; excitatory synapse; extracellular space; integral to membrane; integral to plasma membrane; plasma membrane; postsynaptic density; postsynaptic membrane; synapse

Molecular Function: cell adhesion molecule binding; chloride ion binding; neurexin binding; protein binding; protein homodimerization activity; receptor activity

Biological Process: adult behavior; cerebellum development; intercellular junction assembly and maintenance; learning; neuron adhesion; neuron differentiation; organ growth; social behavior; synapse organization and biogenesis; synaptic vesicle endocytosis

Disease: Asperger Syndrome, X-linked, Susceptibility To, 2; Autism, Susceptibility To, X-linked 2