Typical Testing Data/Standard Curve (for reference only)
Human Solute carrier family 12 member 2 ELISA Kit | NKCC-1 elisa kit
Human Solute carrier family 12 member 2 ELISA Kit
Gene Names
Slc12a2; Nkcc1; mBSC2; sy-ns; 9330166H04Rik
Reactivity
Human
Synonyms
Solute carrier family 12 member 2; Human Solute carrier family 12 member 2 ELISA Kit; NKCC-1 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of SLC12A2. No significant cross-reactivity or interference between SLC12A2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SLC12A2 and all the analogues, therefore, cross reaction may still exist in some cases.
Sequence Length
1206
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NKCC-1 elisa kit
Intended Uses: This SLC12A2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SLC12A2. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: SLC12A2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SLC12A2 antibody and an SLC12A2-HRP conjugate. The assay sample and buffer are incubated together with SLC12A2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLC12A2 concentration since SLC12A2 from samples and SLC12A2-HRP conjugate compete for the anti-SLC12A2 antibody binding site. Since the number of sites is limited, as more sites are occupied by SLC12A2 from the sample, fewer sites are left to bind SLC12A2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLC12A2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: SLC12A2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SLC12A2 antibody and an SLC12A2-HRP conjugate. The assay sample and buffer are incubated together with SLC12A2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SLC12A2 concentration since SLC12A2 from samples and SLC12A2-HRP conjugate compete for the anti-SLC12A2 antibody binding site. Since the number of sites is limited, as more sites are occupied by SLC12A2 from the sample, fewer sites are left to bind SLC12A2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SLC12A2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
131,034 Da
NCBI Official Full Name
solute carrier family 12 member 2
NCBI Official Synonym Full Names
solute carrier family 12, member 2
NCBI Official Symbol
Slc12a2
NCBI Official Synonym Symbols
Nkcc1; mBSC2; sy-ns; 9330166H04Rik
NCBI Protein Information
solute carrier family 12 member 2
UniProt Protein Name
Solute carrier family 12 member 2
Protein Family
UniProt Gene Name
Slc12a2
UniProt Synonym Gene Names
Nkcc1
Uniprot Description
Electrically silent transporter system. Mediates sodium and chloride reabsorption. Plays a vital role in the regulation of ionic balance and cell volume.
Research Articles on NKCC-1
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Product Notes
The Human NKCC-1 slc12a2 (Catalog #AAA7254348) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7254348 ELISA Kit recognizes Human NKCC-1. It is sometimes possible for the material contained within the vial of "Solute carrier family 12 member 2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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