Nuclear Factor I/X Recombinant Protein | NFIX recombinant protein

Recombinant Nuclear Factor I/X (NFIX)

Cat. Num. AAA2033302

• Gene Names: NFIX; NF1A; MRSHSS; SOTOS2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >90%
• Synonyms: Nuclear Factor I/X; Recombinant Nuclear Factor I/X (NFIX); NF1A; CTF; CCAAT-Binding Transcription Factor; TGGCA-binding protein; NFIX recombinant protein

• Host: E Coli
• Purity/Purification: >90%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: HPFIEALL PHVRAFSYTW FNLQARKRKY FKKHEKRMSKDEERAVKDEL LGEKPEIKQK WASRLLAKLR KDIRPEFRED FVLTITGKKPPCCVLSNPDQ KGKIRRIDCL RQADKVWRLD LVMVILFKGI PLESTDGERLYKSPQCSNPG LCVQPHHIGV TIKELDLYLA YFVHTPESGQ SDSSNQQGDADIKPLPNGHL SFQDCFVTSG VWNVTELVRV SQTPVATASG PNFSLADLESPSYYNINQVT LGRRSITSPP STSTTKRPKS IDDSEMESPV DDVFYPGT
• Sequence Length: 255

• Applicable Applications for NFIX recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 62.5kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: His13~Thr298
• Organism Species: Rat
• Tag: two N-terminal Tags, His-tag and GST-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 5042399
• NCBI GeneID: 4784
• Molecular Weight: 47,911 Da
• NCBI Official Full Name: nuclear factor I-X, partial
• NCBI Official Synonym Full Names: nuclear factor I/X (CCAAT-binding transcription factor)
• NCBI Official Symbol: NFIX
• NCBI Official Synonym Symbols: NF1A; MRSHSS; SOTOS2
• NCBI Protein Information: nuclear factor 1 X-type
• UniProt Protein Name: Nuclear factor 1 X-type
• Protein Family: Nuclear factor
• UniProt Gene Name: NFIX
• UniProt Synonym Gene Names: NF1-X; Nuclear factor 1/X; CTF; NF-I/X; NFI-X
• UniProt Entry Name: NFIX_HUMAN

NCBI Description

The protein encoded by this gene is a transcription factor that binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3 in viral and cellular promoters. The encoded protein can also stimulate adenovirus replication in vitro. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2012]

Uniprot Description

NFI-X: Recognizes and binds the palindromic sequence 5'- TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication. Defects in NFIX are the cause of Sotos syndrome 2 (SOTOS2). A form of Sotos syndrome, a childhood overgrowth syndrome characterized by pre- and postnatal overgrowth, developmental delay, mental retardation, advanced bone age, and abnormal craniofacial morphology. SOTOS2 patients have macrocephaly, long narrow face, high forehead, slender habitus, scoliosis, and unusual behavior characterized especially by anxiety. Defects in NFIX are the cause of Marshall-Smith syndrome (MRSHSS). A distinct malformation syndrome characterized by accelerated skeletal maturation, relative failure to thrive, respiratory difficulties, mental retardation, and unusual facies, including prominent forehead, shallow orbits, blue sclerae, depressed nasal bridge, and micrognathia. Additional skeletal findings include long and thin tubular bones, broad middle phalanges with relatively narrow distal phalanges, and scoliosis. Belongs to the CTF/NF-I family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleus

Molecular Function: DNA binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding; transcription factor activity

Biological Process: astrocyte differentiation; cerebellar granule cell differentiation; cerebellar Purkinje cell layer development; DNA replication; negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter

Disease: Marshall-smith Syndrome; Sotos Syndrome 2