NEIL1 recombinant protein

Recombinant Human NEIL1 protein

Cat. Num. AAA1562281

• Gene Names: NEIL1; FPG1; NEI1; hFPG1
• Purity: >90% as determined by SDS-PAGE
• Synonyms: NEIL1; Recombinant Human NEIL1 protein; FPG1 Protein; Human; hFPG1 Protein; NEI1 Protein; NEIL1 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human NEIL1 (Ser43-Ala314) was fused with His tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for NEIL1 recombinant protein

NEIL1 is a member of DNA glycosylases. DNA glycosylases are a family homologous to the bacterial Fpg/Nei family. They play a role in base excision repair which is the mechanism by which damaged bases in DNA are removed and replaced. The first step of this process is catalyzed by DNA glycosylases. They remove the damaged nitrogenous base while leaving the sugar-phosphate backbone intact, creating an apurinic/apyrimidinic site, commonly referred to as an AP site. NEIL1 functions in base excision repair of DNA damaged by oxidation or by mutagenic agents. It acts as DNA glycosylase that recognizes and removes damaged bases. NEIL1 prefers to oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. It has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand and cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.

Product Categories/Family for NEIL1 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 375065857
• NCBI GeneID: 79661
• NCBI Accession #: NP_001243481.1
• NCBI GenBank Nucleotide #: NM_001256552.1
• UniProt Accession #: Q96FI4; Q6ZRA7; Q86XW7; Q9H6C3; D3DW75
• Molecular Weight: 43,684 Da
• NCBI Official Full Name: endonuclease 8-like 1 isoform 1
• NCBI Official Synonym Full Names: nei endonuclease VIII-like 1 (E. coli)
• NCBI Official Symbol: NEIL1
• NCBI Official Synonym Symbols: FPG1; NEI1; hFPG1
• NCBI Protein Information: endonuclease 8-like 1; NEH1; nei homolog 1; endonuclease VIII; nei-like protein 1; endonuclease VIII-like 1; DNA glycosylase/AP lyase Neil1; DNA-(apurinic or apyrimidinic site) lyase Neil1
• UniProt Protein Name: Endonuclease 8-like 1
• Protein Family: Endonuclease
• UniProt Gene Name: NEIL1
• UniProt Synonym Gene Names: NEH1
• UniProt Entry Name: NEIL1_HUMAN

NCBI Description

This gene is a member of the Nei endonuclease VIII-like gene family which encodes DNA glycosylases. The encoded enzyme participates in the DNA repair pathway by initiating base excision repair by removing damaged bases, primarily oxidized pyrimidines. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]

Uniprot Description

NEIL1: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8- oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Belongs to the FPG family.

Protein type: DNA repair, damage; Lyase; EC 4.2.99.18; Hydrolase

Chromosomal Location of Human Ortholog: 15q24.2

Cellular Component: nucleoplasm; cytoplasm; microtubule organizing center; chromosome; nucleus

Molecular Function: protein C-terminus binding; DNA-(apurinic or apyrimidinic site) lyase activity; zinc ion binding; DNA N-glycosylase activity; damaged DNA binding; hydrolase activity, acting on glycosyl bonds

Biological Process: base-excision repair; nucleotide-excision repair; response to oxidative stress; DNA catabolic process, endonucleolytic; negative regulation of nuclease activity