HEC1 recombinant protein

Recombinant Human HEC1 protein

Cat. Num. AAA1561194

• Gene Names: NDC80; HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80
• Purity: >90% as determined by SDS-PAGE
• Synonyms: HEC1; Recombinant Human HEC1 protein; Kinetochore protein NDC80 homolog; Highly expressed in cancer protein; Kinetochore protein Hec1; HsHec1; Kinetochore-associated protein 2; Retinoblastoma-associated protein HEC; HEC1 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human HEC1 (Pro290-Glu642) was fused with His tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for HEC1 recombinant protein

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules.

Product Categories/Family for HEC1 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 5174457
• NCBI GeneID: 10403
• NCBI Accession #: NP_006092.1
• NCBI GenBank Nucleotide #: NM_006101.2
• UniProt Accession #: O14777; Q6PJX2
• Molecular Weight: 73,913 Da
• NCBI Official Full Name: kinetochore protein NDC80 homolog
• NCBI Official Synonym Full Names: NDC80 kinetochore complex component
• NCBI Official Symbol: NDC80
• NCBI Official Synonym Symbols: HEC; HEC1; TID3; KNTC2; HsHec1; hsNDC80
• NCBI Protein Information: kinetochore protein NDC80 homolog; kinetochore associated 2; kinetochore protein Hec1; kinetochore-associated protein 2; highly expressed in cancer protein; retinoblastoma-associated protein HEC; NDC80 kinetochore complex component homolog; NDC80 homolog,
• UniProt Protein Name: Kinetochore protein NDC80 homolog
• Protein Family: Transcription factor
• UniProt Gene Name: NDC80
• UniProt Synonym Gene Names: HEC; HEC1; KNTC2; HsHec1
• UniProt Entry Name: NDC80_HUMAN

NCBI Description

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011]

Uniprot Description

HEC1: plays an essential role in chromosome segregation by interacting through its coiled-coil domains with several proteins that modulate the G2/M phase. Phosphorylation by Nek2 is essential for faithful chromosome segregation. Required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 18p11.32

Cellular Component: kinetochore; membrane; cytosol; nucleus; chromosome, pericentric region

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: mitosis; mitotic spindle organization and biogenesis; establishment of mitotic spindle orientation; mitotic sister chromatid segregation; attachment of spindle microtubules to kinetochore during mitosis; cell division; mitotic cell cycle; attachment of spindle microtubules to kinetochore; chromosome segregation