Nicastrin Recombinant Protein | NCSTN recombinant protein

Nicastrin Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123038

• Gene Names: Ncstn; Nct; Aph2; AA727311; D1Dau13e; Kiaa0253; mKIAA0253; 9430068N19Rik
• Purity: >99% as determined by SDS-PAGE
• Synonyms: Nicastrin; Nicastrin Protein; Mouse; Recombinant (His Tag); Mouse Nicastrin/NCSTN Protein (His Tag); 9430068N19Rik Protein; AA727311 Protein; Aph2 Protein; D1Dau13e Protein; Kiaa0253 Protein; mKIAA0253 Protein; NCSTN Protein; Nct Protein; nicastrin; NCSTN recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >99% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln668

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Gly 28
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse NCSTN (P57716)(Met1-Gln668) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for NCSTN recombinant protein

Background: Nicastrin (NCST, or NCT), a single-pass membrane glycoprotein that harbors a large extracellular domain, is an essential component of the gamma-secretase complex. Several lines of evidence indicate that the members of these complexes could also contribute to the control of cell death. NCT controls cell death via phosphoinositide 3-kinase/Akt and p53-dependent pathways and that this function remains independent of the activity and molecular integrity of the gamma-secretase complexes. Increasing pieces of evidence have shown that Nicastrin/NCSTN plays a crucial role in gamma-cleavage of the amyloid precursor protein (APP). The glycoprotein Nicastrin is an essential component of the gamma-secretase complex, a high molecular weight complex that also contains the presenilin proteins, Aph-1 and Pen-2. The gamma-secretase complex is not only involved in APP processing but also in the processing of an increasing number of another type I integral membrane proteins. As the largest subunit of the gamma-secretase complex, Nicastrin plays a crucial role in its activation. Inhibition of NCSTN demonstrated an altered gamma-cleavage activity, suggesting its potential implication in developing Alzheimer's disease (AD). Besides, Nicastrin can function to maintain epithelial to mesenchymal transition during breast cancer progression. Anti-nicastrin polyclonal and monoclonal antibodies were able to decrease notch1 and vimentin expression and reduced the invasive capacity of breast cancer cells in vitro.

References

He G, et al. (2007) Degradation of nicastrin involves both proteasome and lysosome. J Neurochem. 101(4): 982-92.Hayashi I, et al. (2009) Single chain variable fragment against nicastrin inhibits the gamma-secretase activity. J Biol Chem. 284(41): 27838-47.Ma Z, et al. (2009) Association between promoter polymorphisms of the nicastrin gene and sporadic Alzheimer's disease in North Chinese Han population. Neurosci Lett. 458(3): 136-9.Pardossi-Piquard R, et al. (2009) p53-dependent control of cell death by nicastrin: lack of requirement for presenilin-dependent gamma-secretase complex. J Neurochem. 109(1): 225-37.Filipovi A, et al. (2011) Biological and clinical implications of nicastrin expression in invasive breast cancer. Breast Cancer Res Treat. 125(1): 43-53.

NCBI and Uniprot Product Information

• NCBI GI #: 224809376
• NCBI GeneID: 59287
• NCBI Accession #: NP_067620.3
• NCBI GenBank Nucleotide #: NM_021607.3
• UniProt Accession #: P57716; Q8VE20; E9QLZ6
• Molecular Weight: 78,492 Da
• NCBI Official Full Name: nicastrin
• NCBI Official Synonym Full Names: nicastrin
• NCBI Official Symbol: Ncstn
• NCBI Official Synonym Symbols: Nct; Aph2; AA727311; D1Dau13e; Kiaa0253; mKIAA0253; 9430068N19Rik
• NCBI Protein Information: nicastrin
• UniProt Protein Name: Nicastrin
• Protein Family: Nicastrin
• UniProt Gene Name: Ncstn
• UniProt Entry Name: NICA_MOUSE