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Human Putative neutrophil cytosol factor 1C ELISA Kit | NCF1C elisa kit

Human Putative neutrophil cytosol factor 1C (NCF1C) ELISA Kit

Gene Names
NCF1C; SH3PXD1C
Reactivity
Human
Synonyms
Putative neutrophil cytosol factor 1C; Human Putative neutrophil cytosol factor 1C (NCF1C) ELISA Kit; NCF1C elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human NCF1C. No significant cross-reactivity or interference between Human NCF1C and analogues was observed.
Sequence Length
366
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for NCF1C elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate NCF1C in samples. An antibody specific for NCF1C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNCF1C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NCF1C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NCF1C bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
41,851 Da
NCBI Official Full Name
Putative neutrophil cytosol factor 1C
NCBI Official Synonym Full Names
neutrophil cytosolic factor 1C pseudogene
NCBI Official Symbol
NCF1C
NCBI Official Synonym Symbols
SH3PXD1C
UniProt Protein Name
Putative neutrophil cytosol factor 1C
UniProt Gene Name
NCF1C
UniProt Synonym Gene Names
SH3PXD1C; NCF-1C

NCBI Description

The neutrophil cytosolic factor 1 (NCF1) gene encodes the 47 kDa cytosolic subunit of neutrophil NADPH oxidase, which produces superoxide anion. The NCF1 gene is located in close proximity to two highly similar, multi-exon pseudogenes at chromosome 7q11.23, corresponding to this gene record and GeneID:654816. The two pseudogenes contain a dinucleotide deletion (delta-GT) in exon 2 that results in a frameshift and truncation of the open reading frame, and neither pseudogene is likely to express a protein. Recombination events between the pseudogenes and the functional NCF1 gene can inactivate the NCF1 gene and result in chronic granulomatous disease. [provided by RefSeq, Nov 2009]

Uniprot Description

May be required for activation of the latent NADPH oxidase (necessary for superoxide production).

Research Articles on NCF1C

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Product Notes

The Human NCF1C ncf1c (Catalog #AAA280394) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA280394 ELISA Kit recognizes Human NCF1C. It is sometimes possible for the material contained within the vial of "Putative neutrophil cytosol factor 1C, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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