NCF1 recombinant protein

Recombinant Human NCF1 Protein

Cat. Num. AAA8249480

• Gene Names: NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: NCF1; Recombinant Human NCF1 Protein; NOXO2; SH3PXD1A; Neutrophil cytosol factor 1; NCF-1; 47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; NCF-47K; Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; SH3 and PX domain-containing protein 1A; p47-phox; NCF1 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM Tris, 1mM DTT, 0.1M NaCl, pH 8.0.
• Sequence: MGDTFIRHIA LLGFEKRFVP SQHYVYMFLV KWQDLSEKVV YRRFTEIYEF HKTLKEMFPI EAGAINPENR IIPHLPAPKW FDGQRAAENR QGTLTEYCST LMSLPTKISR CPHLLDFFKV RPDDLKLPTD NQTKKPETYL MPKDGKSTAT DITGPIILQT YRAIANYEKT SGSEMALSTG DVVEVVEKSE SGWWFCQMKA KRGWIPASFL EPLDSPDETE DPEPNYAGEP YVAIKAYTAV EGDEVSLLEG EAVEVIHKLL DGWWVIRKDD VTGYFPSMYL QKSGQDVSQA QRQIKRGAPP RRSSIRNAHS IHQRSRKRLS QDAYRRNSVR FLQQRRRQAR PGPQSPGSPL EEERQTQRSK PQPAVPPRPS ADLILNRCSE STKRKLASAV EHHHHHH
• Sequence Length: 390

• Applicable Applications for NCF1 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for NCF1 recombinant protein

Recombinant Human NCF1 Protein is produced by our E Coli expression system and the target gene encoding Met1-Val390 is expressed.

NCBI and Uniprot Product Information

• NCBI GI #: 528078313
• NCBI GeneID: 653361
• NCBI Accession #: NP_000256.4
• NCBI GenBank Nucleotide #: NM_000265.5
• UniProt Accession #: P14598; O43842; Q2PP07; Q53FR5; Q9BU90; Q9BXI7; Q9BXI8; Q9UDV9; Q9UMU2; A6NEH2; A8K7S9
• Molecular Weight: 30,781 Da
• NCBI Official Full Name: neutrophil cytosol factor 1
• NCBI Official Synonym Full Names: neutrophil cytosolic factor 1
• NCBI Official Symbol: NCF1
• NCBI Official Synonym Symbols: NCF1A; NOXO2; p47phox; SH3PXD1A
• NCBI Protein Information: neutrophil cytosol factor 1
• UniProt Protein Name: Neutrophil cytosol factor 1
• Protein Family: Neutrophil cytosol factor
• UniProt Gene Name: NCF1
• UniProt Synonym Gene Names: NOXO2; SH3PXD1A; NCF-1
• UniProt Entry Name: NCF1_HUMAN

NCBI Description

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]

Uniprot Description

p47phox: the 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. The holo-oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Contains 2 SH2 domains. Mutations in NCF1, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease.

Protein type: EC 1.-.-.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: cytosol; extrinsic to membrane; NADPH oxidase complex

Molecular Function: electron carrier activity; phosphatidylinositol-3,4-bisphosphate binding; phosphoinositide binding; protein binding; SH3 domain binding; superoxide-generating NADPH oxidase activator activity; superoxide-generating NADPH oxidase activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; cell redox homeostasis; cellular defense response; innate immune response; protein targeting to membrane; respiratory burst; superoxide metabolic process; superoxide release; vascular endothelial growth factor receptor signaling pathway

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type I