Nuclear cap-binding protein subunit 1 (NCBP1) Recombinant Protein | NCBP1 recombinant protein

Recombinant Chicken Nuclear cap-binding protein subunit 1 (NCBP1), partial

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Nuclear cap-binding protein subunit 1 (NCBP1); Recombinant Chicken Nuclear cap-binding protein subunit 1 (NCBP1); partial; NCBP1 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 793

• Species: Chicken
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

Related Product Information for NCBP1 recombinant protein

The product of this gene is a component of the nuclear cap-binding protein complex (CBC), which binds to the monomethylated 5 cap of nascent pre-mRNA in the nucleoplasm. The encoded protein promotes high-affinity mRNA-cap binding and associates with the CTD of RNA polymerase II. The CBC promotes pre-mRNA splicing, 3 -end processing, RNA nuclear export, and nonsense-mediated mRNA decay.

NCBI and Uniprot Product Information

• NCBI GI #: 71895341
• NCBI GeneID: 427379
• NCBI Accession #: NP_001026611.1
• NCBI GenBank Nucleotide #: NM_001031440.1
• UniProt Accession #: Q5ZJZ6
• Molecular Weight: 92,564 Da
• NCBI Official Full Name: nuclear cap-binding protein subunit 1
• NCBI Official Synonym Full Names: nuclear cap binding protein subunit 1
• NCBI Official Symbol: NCBP1
• NCBI Protein Information: nuclear cap-binding protein subunit 1
• UniProt Protein Name: Nuclear cap-binding protein subunit 1
• UniProt Gene Name: NCBP1
• UniProt Synonym Gene Names: CBP80; CBP80; NCBP 80 kDa subunit

Uniprot Description

Component of the cap-binding complex (CBC), which binds cotranscriptionally to the 5'-cap of pre-mRNAs and is involved in various processes such as pre-mRNA splicing, translation regulation, nonsense-mediated mRNA decay, RNA-mediated gene silencing (RNAi) by microRNAs (miRNAs) and mRNA export. The CBC complex is involved in mRNA export from the nucleus, leading to the recruitment of the mRNA export machinery to the 5'-end of mRNA and to mRNA export in a 5' to 3' direction through the nuclear pore. The CBC complex is also involved in mediating U snRNA and intronless mRNAs export from the nucleus. The CBC complex is essential for a pioneer round of mRNA translation, before steady state translation when the CBC complex is replaced by cytoplasmic cap-binding protein eIF4E. The pioneer round of mRNA translation mediated by the CBC complex plays a central role in nonsense-mediated mRNA decay (NMD), NMD only taking place in mRNAs bound to the CBC complex, but not on eIF4E-bound mRNAs. The CBC complex enhances NMD in mRNAs containing at least one exon-junction complex (EJC), promoting the interaction between UPF1 and UPF2. The CBC complex is also involved in 'failsafe' NMD, which is independent of the EJC complex, while it does not participate in Staufen-mediated mRNA decay (SMD). During cell proliferation, the CBC complex is also involved in microRNAs (miRNAs) biogenesis via its interaction with SRRT/ARS2 and is required for miRNA-mediated RNA interference. The CBC complex also acts as a negative regulator of PARN, thereby acting as an inhibitor of mRNA deadenylation. In the CBC complex, NCBP1/CBP80 does not bind directly capped RNAs (m7GpppG-capped RNA) but is required to stabilize the movement of the N-terminal loop of NCBP2/CBP20 and lock the CBC into a high affinity cap-binding state with the cap structure. Associates with NCBP3 to form an alternative cap-binding complex (CBC) which plays a key role in mRNA export. The conventional CBC with NCBP2 binds both small nuclear RNA (snRNA) and messenger (mRNA) and is involved in their export from the nucleus whereas the alternative CBC with NCBP3 does not bind snRNA and associates only with mRNA thereby playing a role only in mRNA export ().