Apolipoprotein E, Western Blot Control Native Protein
Apolipoprotein E, Human, Western Blot Control (Apo E)
Applications
Western Blot
Purity
PurifiedPurified
Synonyms
Apolipoprotein E; Western Blot Control; Human; Western Blot Control (Apo E); Western Blot Control native protein
Purity/Purification
Purified
Purified
Purified
Form/Format
Supplied as a liquid in SDS-PAGE sample buffer (reduced) for direct loading on gels (10ul/lane). It contains a mixture of various ApoE isoforms.
Applicable Applications for Apolipoprotein E, Western Blot Control native protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot as a positive control.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Apolipoprotein E, Western Blot Control native protein
Apolipoprotein E (apo E), a component of lipoproteins produced by the liver and in circulating macrophages, plays a critical role in the reverse transport of cholesterol to the liver via the circulation. ApoE is also expressed in the brain and in response to injury in both the peripheral and central nervous systems. ApoE is a single polypeptide chain of 299 amino acids (~34kD) consisting of two independently folded functional domains. The C-terminal domain contains the major lipid-binding region. The N-terminal domain exists in the lipid-free state as a four-helix bundle of amphipathic a-helices and contains the LDLR-binding region (amino acids 136-150 in helix), which coincides with a heparin-binding site. Humans and mice lacking apoE cannot clear remnant lipoproteins from the plasma and are at increased risk for atherosclerosis. ApoE is a high-affinity ligand for the low density lipoprotein receptor (LDLR) family and for cell-surface heparan sulfate proteoglycans. Defective binding of apoE to receptors causes cholesterol-rich lipoprotein particles to accumulate in the plasma and is the mechanism of type III hyperlipoproteinemia, a genetic disorder characterized by elevated plasma cholesterol and triglyceride levels and accelerated coronary artery disease. In humans, apoE has three major isoforms: Apo E2 (Cys112, Cys158), Apo E3 (Cys112, Arg158), Apo E4 (Arg112, Arg158), products of alleles at a single gene locus. Although apoE4 is neither necessary nor sufficient to cause AD, inheritance of apoE4 is a significant risk factor for late onset AD, decreasing the age of onset and the duration of disease. Due primarily to this genetic linkage, the role of apoE in the pathogenesis AD is being actively studied.
Product Categories/Family for Apolipoprotein E, Western Blot Control native protein
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Product Notes
The Apolipoprotein E, Western Blot Control (Catalog #AAA656896) is a Native Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Apolipoprotein E, Western Blot Control can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot as a positive control. Researchers should empirically determine the suitability of the Apolipoprotein E, Western Blot Control for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Apolipoprotein E, Western Blot Control, Native Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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