Nitrate reductase molybdenum cofactor assembly chaperone NarJ (narJ) Recombinant Protein | narJ recombinant protein
Recombinant Escherichia coli Nitrate reductase molybdenum cofactor assembly chaperone NarJ (narJ)
NCBI and Uniprot Product Information
NCBI Description
NarJ has two NarG binding sites: one for Mo insertion and one to block membrane attachment via NarI until activation is complete. [More information is available at EcoGene: EG10641]. NarJ is parto of the redox enzyme maturation protein (REMP) family of chaperones . [More information is available at EcoCyc: EG10641].
Uniprot Description
Chaperone required for proper molybdenum cofactor insertion and final assembly of the membrane-bound respiratory nitrate reductase 1. Required for the insertion of the molybdenum into the apo-NarG subunit, maybe by keeping NarG in an appropriate competent-open conformation for the molybdenum cofactor insertion to occur. NarJ maintains the apoNarGH complex in a soluble state. Upon insertion of the molybdenum cofactor, NarJ seems to dissociate from the activated soluble NarGH complex, before its association with the NarI subunit on the membrane.
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Product Notes
The narJ narj (Catalog #AAA1122802) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-236, full length protein. The amino acid sequence is listed below: MIELVIVSRL LEYPDAALWQ HQQEMFEAIA ASKNLPKEDA HALGIFLRDL TTMDPLDAQA QYSELFDRGR ATSLLLFEHV HGESRDRGQA MVDLLAQYEQ HGLQLNSREL PDHLPLYLEY LAQLPQSEAV EGLKDIAPIL ALLSARLQQR ESRYAVLFDL LLKLANTAID SDKVAEKIAD EARDDTPQAL DAVWEEEQVK FFADKGCGDS AITAHQRRFA GAVAPQYLNI TTGGQH. It is sometimes possible for the material contained within the vial of "Nitrate reductase molybdenum cofactor assembly chaperone NarJ (narJ), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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