NAPA recombinant protein

NAPA, 1-295aa, Human, His tag, E Coli

Cat. Num. AAA203500

• Gene Names: NAPA; SNAPA
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: NAPA; 1-295aa; Human; His tag; E Coli; N-ethylmaleimide-sensitive factor attachment protein alpha; SNAPA; SNAP-alpha; NAPA recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20 mM Tris-HCl Buffer (pH 7.5) containing 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MDNSGKEAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN MFKMAKNWSA AGNAFCQAAQ LHLQLQSKHD AATCFVDAGN AFKKADPQEA INCLMRAIEI YTDMGRFTIA AKHHISIAEI YETELVDIEK AIAHYEQSAD YYKGEESNSS ANKCLLKVAG YAALLEQYQK AIDIYEQVGT NAMDSPLLKY SAKDYFFKAA LCHFCIDMLN AKLAVQKYEE LFPAFSDSRE CKLMKKLLEA HEEQNVDSYT ESVKEYDSIS RLDQWLTTML LRIKKTIQGD EEDLR
• Sequence Length: 295

• Applicable Applications for NAPA recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for NAPA recombinant protein

NAPA is one of the SNAP (Soluble NSF Attachment Protein) family. SNAPs, acting in concert with SNAREs (SNAP receptors) and the N-ethylmaleimide-sensitive fusion protein (NSF), are required for the fusion of transport vesicles to their target membranes in synaptic transmission, intra-Golgi transport, endosome-to-endosome fusion and transcytotic vesicles-to-plasma membrane transport. This protein mediates the binding of NSF and thus the formation of a 20S fusion particle. It is expressed in all mammalian tissues. Recombinant human NAPA protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography.

Product Categories/Family for NAPA recombinant protein

Neuroscience

References

Thummel CS., et al. (2010) Dev Dyn. 239 (3):954-64; Lee JS., et al (2002) J Basic Microbiol. 42 (4):238-45.;

NCBI and Uniprot Product Information

• NCBI GI #: 47933379
• NCBI GeneID: 8775
• NCBI Accession #: NP_003818
• NCBI GenBank Nucleotide #: NM_003827.3
• UniProt Accession #: P54920; Q96IK3; Q9BVJ3; A8K879
• Molecular Weight: 35.3 kDa (315aa), confirmed by MALDI-TOF
• NCBI Official Full Name: alpha-soluble NSF attachment protein
• NCBI Official Synonym Full Names: NSF attachment protein alpha
• NCBI Official Symbol: NAPA
• NCBI Official Synonym Symbols: SNAPA
• NCBI Protein Information: alpha-soluble NSF attachment protein
• UniProt Protein Name: Alpha-soluble NSF attachment protein
• Protein Family: Na(+)/H(+) antiporter
• UniProt Gene Name: NAPA
• UniProt Synonym Gene Names: SNAPA; SNAP-alpha

NCBI Description

This gene encodes a member of the soluble NSF attachment protein (SNAP) family. SNAP proteins play a critical role in the docking and fusion of vesicles to target membranes as part of the 20S NSF-SNAP-SNARE complex. The encoded protein plays a role in the completion of membrane fusion by mediating the interaction of N-ethylmaleimide-sensitive factor (NSF) with the vesicle-associated and membrane-associated SNAP receptor (SNARE) complex, and stimulating the ATPase activity of NSF. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus (Probable). Together with GNA12 promotes CDH5 localization to plasma membrane (PubMed:15980433).