NAPA recombinant protein

Recombinant Human NAPA Protein

Cat. Num. AAA8249478

• Gene Names: NAPA; SNAPA
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: NAPA; Recombinant Human NAPA Protein; SNAPA; Alpha-soluble NSF attachment protein; SNAP-alpha; N-ethylmaleimide-sensitive factor attachment protein alpha; NAPA recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM Tris, 150mM NaCl, pH 8.0.
• Sequence: MGSSHHHHHH SSGLVPRGSH MDNSGKEAEA MALLAEAERK VKNSQSFFSG LFGGSSKIEE ACEIYARAAN MFKMAKNWSA AGNAFCQAAQ LHLQLQSKHD AATCFVDAGN AFKKADPQEA INCLMRAIEI YTDMGRFTIA AKHHISIAEI YETELVDIEK AIAHYEQSAD YYKGEESNSS ANKCLLKVAG YAALLEQYQK AIDIYEQVGT NAMDSPLLKY SAKDYFFKAA LCHFCIDMLN AKLAVQKYEE LFPAFSDSRE CKLMKKLLEA HEEQNVDSYT ESVKEYDSIS RLDQWLTTML LRIKKTIQGD EEDLR
• Sequence Length: 295

• Applicable Applications for NAPA recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for NAPA recombinant protein

Recombinant Human NAPA Protein is produced by our E Coli expression system and the target gene encoding Met1-Arg295 is expressed with a 6His tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 47933379
• NCBI GeneID: 8775
• NCBI Accession #: NP_003818.2
• NCBI GenBank Nucleotide #: NM_003827.3
• UniProt Accession #: P54920; Q96IK3; Q9BVJ3; A8K879
• Molecular Weight: 33,233 Da
• NCBI Official Full Name: alpha-soluble NSF attachment protein
• NCBI Official Synonym Full Names: NSF attachment protein alpha
• NCBI Official Symbol: NAPA
• NCBI Official Synonym Symbols: SNAPA
• NCBI Protein Information: alpha-soluble NSF attachment protein
• UniProt Protein Name: Alpha-soluble NSF attachment protein
• Protein Family: Na(+)/H(+) antiporter
• UniProt Gene Name: NAPA
• UniProt Synonym Gene Names: SNAPA; SNAP-alpha
• UniProt Entry Name: SNAA_HUMAN

NCBI Description

This gene encodes a member of the soluble NSF attachment protein (SNAP) family. SNAP proteins play a critical role in the docking and fusion of vesicles to target membranes as part of the 20S NSF-SNAP-SNARE complex. The encoded protein plays a role in the completion of membrane fusion by mediating the interaction of N-ethylmaleimide-sensitive factor (NSF) with the vesicle-associated and membrane-associated SNAP receptor (SNARE) complex, and stimulating the ATPase activity of NSF. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jun 2011]

Uniprot Description

SNAP-alpha: Required for vesicular transport between the endoplasmic reticulum and the Golgi apparatus. Belongs to the SNAP family.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 19q13.33

Cellular Component: cytosol; membrane; SNARE complex; vacuolar membrane

Molecular Function: protein binding; protein complex binding; SNARE binding; soluble NSF attachment protein activity; syntaxin binding

Biological Process: COPII coating of Golgi vesicle; ER to Golgi vesicle-mediated transport; intra-Golgi vesicle-mediated transport; intracellular protein transport; retrograde vesicle-mediated transport, Golgi to ER